About AAV
Advantages | Limitations | Field of Application |
---|---|---|
- Tissue-specific targeting by choice of serotype - Very low immunogenicity - Non-integrative - Potential for long-term gene expression |
- Relatively small packaging capacity (~4.7 kb) - Not useful for making stable cell lines |
Tissue specific long term gene overexpression and knockdown in vivo |
AAV Serotypes
AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to efficiently infect with specificity.
Among them, AAV-DJ is a mix of 8 naturally occurring serotyes and efficient for various types of human tissues and organs.
We will provide AAV viruses with serotype DJ. If you need other serotypes, please inquire.
Service Details
1. Co-transfection of the expression vector with a serotype specific helper plasmids(AAV-DJ) into HEK293T cells.
2. Freeze-thaw cell pellets
3. Iodixanol gradient purification of AAV by ultracentrifugation
4. Dialysis and concentration
5. AAV titer determination by qPCR
Service type | Application | Description |
---|---|---|
Cell lysate | in vitro | Lysates were formed by three freeze-thaw cycles |
Concentrated | in vivo | Virus are distributed as 100 µL solutions of purified viral particles suitable for in vivo use |