Introduction
• We develop stable cell line which is overexpressing target protein
• Customers can choose transfection method between Lentivirus or non-vial methods
• We produce mammalian cell based human recombinant protein.
Applications
• Protein overexpressed cell line (Available for receptor and channel overexpression or enzyme and protein
productions)
• Reporter cell line (Luciferase, fluorescence protein etc.)
Basic methods
• Cloning of target DNA in vectolor
• Viral packaging (optional)
• Transfection to host cell
• Clone isolation by FACS and Thunder
• Clone expansion
List of services
| Service type | Estimated time | Field of Application |
|---|---|---|
| Gene overexpression cell line development | table pool: 8weeks Single clone: 14weeks |
- Report of cell line viability test - 2 cryovials of stable pool cells with 10^6 cells per vial. - Report of cell clone generation - Report of screening of cell clones (FACS results) - Two single-cell-derived stable clones (1×10^6 cells/vial) - CoA report |
| Inducible cell line development | Stable pool: 12weeks Single clone: 18weeks |
- Report of cell line viability test - 2 cryovials of stable pool cells with 10^6 cells per vial. - Report of cell clone generation - Report of screening of cell clones (FACS results) - One single-cell-derived stable clone (1×10^6 cells/vial) - COA report |
※ Personal consultation is required at all stages of the project
