About Lentivirus
| Advantages | Limitations | Field of Application |
|---|---|---|
|
- Integrates into host genome - Infects both dividing and non-dividing cells - Strong and stable transgene expression - Large packaging capacity (~6 kb) - Can be used to make stable cell lines |
- Potential for insertional mutagenesis due to random integration - Titer highly sensitive to viral genome size - Small probability of generating replication competent lentivirus |
Stable cell model generation to analyze long term effects of gene over expression and silencing for dividing and non-dividing cells |
Service Details
1. Co-transfection of the lentiviral expression vector with lentiviral packaging
plasmids
into HEK293T cells
2. Harvesting of the virus-containing supernatant afterwards.
3. Virus concentration by ultracentrifugation
4. Lentivirus titration by qPCR
Lentivirus Packaging Options
| Service type | Application | Description |
|---|---|---|
| Supernatant | in vitro | Unconcentrated supernatant |
| Concentrated | in vivo | Virus are distributed as 50 µL solutions of purified viral particles suitable for in vivo use |
Control Viruses
We can also offer control viruses. The control virus contain genes encoding scramble shRNA and fluorescent proteins such as GFP and mCherry, etc.
