About AAV
| Advantages | Limitations | Field of Application |
|---|---|---|
| - Tissue-specific targeting by choice of serotype - Very low immunogenicity - Non-integrative - Potential for long-term gene expression |
- Relatively small packaging capacity (~4.7 kb) - Not useful for making stable cell lines |
Tissue specific long term gene overexpression and knockdown in vivo |
AAV Serotypes
AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9,
AAV-DJ/8 and AAV-DJ)
enable AAVs to efficiently infect with specificity.
Among them, AAV-DJ is a mix of 8 naturally
occurring
serotyes and efficient for various types of human tissues and organs.
We will provide AAV viruses with
serotype
DJ. If you need other serotypes, please inquire.
Service Details
1. Co-transfection of the expression vector with a serotype specific helper
plasmids(AAV-DJ)
into HEK293T cells.
2. Freeze-thaw cell pellets
3. Iodixanol gradient purification of AAV by
ultracentrifugation
4. Dialysis and concentration
5. AAV titer determination by qPCR
| Service type | Application | Description |
|---|---|---|
| Cell lysate | in vitro | Lysates were formed by three freeze-thaw cycles |
| Concentrated | in vivo | Virus are distributed as 100 µL solutions of purified viral particles suitable for in vivo use |
