- We develop stable cell line which is overexpressing target protein
- Customers can choose transfection method between Lentivirus or non-vial methods
- Protein overexpression cell line (Available for receptor and channel overexpression or enzyme and protein productions)
- Reporter cell line (Luciferase, fluorescence protein etc.)
- Cloning of target DNA in vector
- Viral packaging (optional)
- Transfection to host cell
- Clone isolation by FACS and Thunder
- Clone expansion
| Service type | Estimated time | Included |
|---|---|---|
| Gene overexpression cell line development | Stable pool: 8weeks Single clone: 14weeks |
- Report of cell viability test- Two cryo -vials of stable pool of cells (10^6 cells per vial) Or Two single-cell-derived stable clone (1×10^6 cells/vial)- Report of cell clone generation -Report of screening of cell clones (FACS results) - Two single-cell-derived stable clones (1×10^6 cells/vial) -CoA report |
| Inducible cell line development | Stable pool: 12weeks Single clone: 18weeks |
- Report of cell viability test - Two cryo-vials of stable pool of cells (10^6 cells per vial) Or Two single-cell-derived stable clone (1×10^6 cells/vial) - Report of cell clone generation- Report of screening of cell clones (FACS results) - COA report |
※ Personal consultation is required at all stages of the project
