EDUCATION
2010.03 ~ 2014.08 B.A. in Sookmyung Women’s University
2015.03 ~ KU-KIST Graduate School of Converging Science and Technology, Korea University
RESEARCH EXPERIENCES
2012.01 ~ 2012.12 Undergraduate intern (Prof. Jonghoon Park, Sookmyung Women’s University)
2013.06 ~ 2013.09 52nd National Undergraduate Symposium on Biology (Aging & Senescence)
EXPERIMENTAL TECHNIQUES
Electrophysiology: Cell patch, Slice patch
Imaging: Calcium imaging, Intrinsic optical signal (IOS) imaging
Molecular biology: Cloning, RT-PCR, Western blot, Biotinylation, Co-immunoprecipitation
Cellular biology: Transfection, Microporation
Primary culture: Astrocyte cell culture, Cochlea tissue culture
Stereotaxic surgery (mouse)
Immunohistochemistry, Immunocytochemistry
PUBLICATIONS
Han K, Lee M, Lim HK, Jang MW, Kwon J, Lee CJ, Kim SG, Suh M. Excitation-inhibition imbalance leads to alteration of neuronal coherence and neurovascular coupling under acute stress. J Neurosci. 2020 Oct 20:JN-RM-1553-20. doi: 10.1523/JNEUROSCI.1553-20.2020. PMID: 33087471.
Woo J, Jang MW, Lee J, Koh W, Mikoshiba K, Lee CJ. The molecular mechanism of synaptic activity-induced astrocytic volume transient. J Physiol. 2020 Jul 24. doi: 10.1113/JP279741. PMID: 32706443.
Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ. Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Exp Neurobiol. 2019 Apr;28(2):183-215. doi: 10.5607/en.2019.28.2.183. Epub 2019 Apr 30. PMID: 31138989.
AWARDS
Oral Presentation:
2017 Brain Science Institute Symposium (The novel channel, AUDITORIN discloses a new class of hearing loss, auditory gliopathy)
Poster Presentation:
2018 Glia Meeting of the Korean Society for Glia Research (Human speech perception by AUDITORIN (TMEM43) in cochlear glia)
2019 7th SIPF-SIF International Workshop on: Structure and Function of Ion Channels and Transporters (AUDITORIN (TMEM43), A Novel Passive Conductance Channel of Cochlear Glia is Critical for Maintenance of Speech Discrimination)
EDUCATION
- 2009.03 ~ 2014.03: B.S. in Korea
University, Seoul, Republic of Korea
- 2015.03 ~ 2022.08: Ph.D course in KU-KIST Graduate School of
Converging Science and Technology, Korea University, Seoul, Republic of Korea
ACADEMIC RESEARCH INTEREST
- Neuroscience (Neuron-glia
interaction)
- Neurodegenerative diseases
- Systemic inflammation
- Reactive oxygen species
(ROS)
-Drug development
- Post-traumatic stress disorder (PTSD)
- Emotions
EXPERIMENTAL TECHNIQUES
- Electrophysiology: Cell patch, Slice
patch
- Imaging: Calcium imaging, Neurotransmitters, sensor probe
- Immunohistochemistry,
Immunocytochemistry
- Molecular biology: Cloning, qRT-PCR, shRNA
- Cellular biology: Transfection,
Microporation
- Astrocyte cell culture
- Behavior test (mouse)
- Stereotaxic surgery (mouse, rat):
virus injection, cannula surgery
- Drug screening
assay (H2O2, MAOB etc.)
- RNA-Sequencing
AWARD and HONORS
■ Oral Presentation award
- 2019 Korean Chemical Society, Division of Medicinal Chemistry; Best Presentation
■ Poster Presentation award
- 2018 Glia Meeting of the Korean Society for Glia Research
- 2019 Cold Spring Harbor Asia (CSHA) meeting, Francis Crick Symposium-Transforming Neurosciences:
Questions & Experiments
- 2021 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation Award
- 2022 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation
Award
- 2022 IBS-UNIST Symposium; Best Presentation Award
PATENTS
■ Composition for prevention or treatment of
rheumatoid arthritis (10-2020-0120924)
■ NOVEL AMINOAROMATIC COMPOUNDS OR
PHARMACEUTICALLY ACCEPTABLE SALT THEREOF AND PHARMACEUTICAL COMPOSITION FOR PREVENTION OR TREATMENT OF
NEURODEGENERATIVE DISEASES COMPRISING THE SAME AS AN ACTIVE INGREDIENT (10-2021-0155076)
■ Prolonging lifespan with long-term administration of novel H2O2 scavenger (10-2022-0092661)
PUBLICATION
1. Won W, Choi HJ, Yoo JY, Kim D, Kim TY, Ju YH, Park KD, Lee H, Jung SY, Lee CJ. inhibiting peripheral and central MAO-B ameliorates both joint inflammation and cognitive impairment in rheumatoid arthritis. Exp Mol Med (in press)
2. An H, Lee H, Yang S, Won W, Lee CJ, Nam MH. Adenovirus-induced Reactive Astrogliosis Exacerbates the Pathology of Parkinson's Disease. Exp Neurobiol. 2021 Jun 30;30(3):222-231. doi: 10.5607/en21013. PMID: 34045369; PMCID: PMC8278136.
3. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim ST, Lee CJ. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med 53, 956–972 (2021).
4. Chun H, Im H, Kang YJ, Kim Y, Shin JH,Won W, Lim J, Ju Y, Park YM, Kim S, Lee SE, Lee J, Woo J, Hwang Y, Cho H, Jo S, Park JH, Kim D, Kim DY, Seo JS, Gwag BJ, Kim YS, Park KD, Kaang BK, Cho H, Ryu H, Lee CJ. Severe reactive astrocytes precipitate pathological hallmarks of Alzheimer's disease via H2O2- production. Nat Neurosci. 2020 Dec;23(12):1555-1566. doi: 10.1038/s41593-020-00735-y. Epub 2020 Nov 16. PMID: 33199896.
5. Nam MH, Cho J, Kwon DH, Park JY, Woo J, Lee JM, Lee S, Ko HY, Won W, Kim RG, Song H, Oh SJ, Choi JW, Park KD, Park EK, Jung H, Kim HS, Lee MC, Yun M, Lee CJ, Kim HI. Excessive Astrocytic GABA Causes Cortical Hypometabolism and Impedes Functional Recovery after Subcortical Stroke.Cell Rep. 2020 Jul 7;32(1):107861. doi: 10.1016/j.celrep.2020.107861. Erratum in: Cell Rep. 2020 Jul 21;32(3):107975. PMID: 32640227.
6. Nam MH*, Won W*, Han KS, Lee CJ. Signaling mechanisms of μ-opioid receptor (MOR) in the hippocampus: disinhibition versus astrocytic glutamate regulation. Cell Mol Life Sci. 2021 Jan;78(2):415-426. doi: 10.1007/s00018-020-03595-8. Epub 2020 Jul 15. PMID: 32671427.
8. Min-Ho Nam, Kyung-Seok Han, Jaekwang Lee, Woojin Won, Wuhyun Koh, Jin Young Bae, Junsung Woo, Jayoung Kim, Elliot Kwong, Tae-Yong Choi, Heejung Chun, Seung Eun Lee, Sang-Bum Kim, Ki Duk Park, Se-Young Choi, Yong Chul Bae, C. Justin Lee. Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference. Cell reports. Volume 28, Issue 5, 30 July 2019, Pages 1154-1166.e5.
- Ph.D course (KU-KIST)
-Glia-neuron interaction, Neurodegeneration and
Neuroinflammation.
-
wwj@ibs.re.kr
Educational background
- 2016/09 ~ Present, Ph.D. candidate in
KU-KIST Graduate School of Converging Science and Technology, Korea University,
Seoul, Republic of Korea (Advisor: C. Justin, Lee)
- 2010/03 ~ 2016/08, B.E. in Biomedical
engineering, Korea University, Seoul, Republic of Korea
- 2012/05 ~ 2014/02, KATUSA soldier program
(U.S. Army Garrison Casey, South Korea)
Research Experiences
- 2014/09 ~ 2016/05, Undergraduate
internship, Prof. Sang-Hoon Lee’s iBML lab, Korea University.
Field of interest
- Targeting
astrocytes as a novel therapeutic target for treating epilepsy.
- Physiological and pathological function
of TRIM9 in astrocytes.
- Modulation of synaptic transmission by
astrocytes.
- Mechanosensitive calcium channels on
radial glial cells.
EXPERIMENTAL TECHNIQUES
- Calcium imaging, GABA imaging
- Behavior test (Passive avoidance test, Open field test, novel
object recognition and novel place recognition test)
- Electrophysiology:
Patch clamping (Cultured cell and acute brain slice), Field recording
- Molecular biology: Cloning, RT-PCR and Western blot
- Primary astrocyte culture
- ICC and IHC
- Virus injection
Publications
-Flow Shear Stress Enhances the
Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation
of Mechanosensitive calcium channel. Park
MG, Jang H, Lee SH, Lee CJ, Exp Neurobiol 2017 Apr;26(2):71-81. (2017)
- Development of a low-cost, comprehensive
recording system for circadian rhythm behavior. Jea Kwon, Min Gu Park, Seung Eun Lee, and C. Justin Lee. Exp
Neurobiol. 2019 Feb;28(1):30-42 (2018)
- Pharmacological Dissection of Intrinsic
Optical Signal Reveals a Functional Coupling between Synaptic Activity and
Astrocytic Volume Transient. Woo J, Han YE, Koh W, Won J, Park MG, An H, Lee CJ, Exp Neurobiol 2018 Feb
27;27(1):65-75; (2018)
- Tweety-homolog (Ttyh) Family Encodes the
Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel
(VRACswell) in the Brain. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang
EM, Jung JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215
(2019)
Awards
- 2017/03 ~ Present, Global Ph.D. Fellowship
program from National Research Foundation of Korea
EDUCATION
-2013.03
~ 2017.08 B.A in Seoul National University
-2017.09
~ Present Ph.D candidate in University of Science and Technology(UST)
RESEARCH
EXPERIENCE
-2016.06
~ 2016.08 Students intern researcher program(학생인턴연구원)
in School of Medicine, Seoul National University.
-2017.03
~ 2017.08 Undergraduate intern in Dr. C. Justin Lee lab, Korea Institute of
Science and Technology (KIST)
EXPERIMENTAL
TECHNIQUES
-Molecular
biology: Cloning, RT-PCR
-Cellular
biology: Transfection, Microporation
-Astrocyte
cell culture (+ Long-term culture)
-Stereotaxic
surgery (mouse)
-Immunohistochemistry
-Imaging:
Calcium imaging in vitro
-Electrophysiology:
Cell patch, Slice patch
-Behavior
test: Passive avoidance test, Open field test, Novel object (Place) recognition
test, Context fear conditioning test, Elevated plus maze test, Forced swim test
AWARDS
-Poster
presentation
2018
Glia Meeting of the Korean Society for Glia Research (Charcterization of
hGFAP-CreERT2 [Tg(GFAP-Cre/ERT2)13Kdmc] in various brain region)
PUBLICATION
-Koh,
W., Park, Y. M., Lee, S. E., & Lee, C. J. (2017). AAV-mediated
astrocyte-specific gene expression under human ALDH1L1 promoter in mouse
thalamus. Experimental neurobiology, 26(6):
350-361.
- Park,
Y. M., Chun, H., Shin, J. I., & Lee, C. J. (2018). Astrocyte Specificity
and Coverage of hGFAP-CreERT2 [Tg (GFAP-Cre/ERT2) 13Kdmc] Mouse Line in Various
Brain Regions. Experimental neurobiology,
27(6): 508-525.