1. Lab information
- Lab Homepage: https://www.ibs.re.kr/glia/
- Contact: Tel. +82-42-878-9155 (Lab)
- Intranet: https://www.ibs.re.kr/glia/
- Contact: PI: C. Justin Lee: Tel. +82-42-878-9150 (Office)/ 010-2825-7128, cjl@ibs.re.kr
2. Be prompt for lab meetings
We have a lab meeting on Mon and a journal club on Fri from 9:00 AM. During this meeting we will discuss lab business and present your own data collected in the past week. Attendance for each meeting is required for all members, please be prompt.
3. Preparing for lab meetings
1) Lab meeting
- - Organizer: Taeyung Kim
- - 9:00 AM, every Monday, C239 seminar room
- - Lab business: notes by Mingu Park
- - Update for mouse status: Jae kyung Kwon
- - Update for virus status: Solji Lee
- - Update for administration: Dasom Kim
- - Full work
- : Presentation for one's recent main project
- : One presenter at a time ordered by age (include Dr. Lee)
- : Prepare as a Ph.D thesis defense for students and as a job talk for postdocs
- - Personal progress
- : Presentation for one's results in past 2 weeks and plans for next 2 weeks
- : Each person is assigned for alternating weeks
- - All files must be saved in the backup folder in ftp://203.247.189.127:4022 (Public NAS) before 9:00 a.m.
2) Journal Club
- - Organizer: Taeyung Kim
- - 9:00 AM, every Friday, C239, C241 seminar room
- - Glance at journals: Yong min Park
: Update the interesting journal articles in current issue from assigned journals
- - Email alert: Yong min Park
: Proposal and selection for the abstract of the most interesting paper for next week
- - Journal club
- : All participants should read the paper thoroughly before the journal club
- : Presenter should prepare a ppt containing introduction, material and method, result (purpose, method, result and implication), and conclusion.
- : Email sender and homepage update for the information of next journal club: Hyunji Kang
- - All files must be saved in the backup folder in ftp://203.247.189.127:4022 (Public NAS) before 9:00 a.m.
4. Keeping the lab clean
Cleaning of your experimental environment is very important for your health as well as quality of the experimental results. Therefore, we may ask you to clean up daily.
In every morning: Right after coming to lab, you may need to check the status of lab bench.
1) Empty waste bottle.
2) Arrangement of all the stuffs in the right place such as pipettes.
3) Check floor and clean if any dirty stuffs.
4) If anything needs to be clean, clean up.
5) Monthly Cleaning:
- - In every Monday of last week after lab meeting, we will clean all equipment and refrigerators. Because of limited space, at this time, you need to clean all the experimental stuffs nearby equipment and make a space in the refrigerators
6) Cell culture room manager
- - Cell line: Minwoo Jang
- - Primary culture: Youngmin Park
- - Cell culture room cleaning every 2 weeks (rotation).
- - Regular cleaning every 3 months.
7) Throw away of waste box by monthly rotation: Jae kyung Kwon makes a list.
8) Throw away of mouse carcass: Jae kyung Kwon.
5. Learning essential skills for experiments
Obtaining basic skills is essential for you to perform experiments properly.
1) Pipetting (training session by Eppendorf representative)
2) Properly calculating molarity and normality
3) Weighing and making stock solutions using volumetric flasks
4) Learn to use experimental machines and equipment
- - Centrifuge (Moonsun Sa)
- - Vortexer and stirrer (Yeonha Ju)
- - pH-meter and osmo-meter (Mingu Park)
- - Sonicator (Wuhyun Koh)
- - Water-bath and heat block (Yeonha Ju)
- - Clean bench and fume hood (Min-woo Jang)
- - Perfusion clean bench and fume hood (Moonsun Sa)
- - Incubator (Joungha Won, Wuhyun Koh, Jae Kwon)
- - Deep freezer (Yongmin Park)
- - Freezer (Each person in charge)
- - Upright Microscopy (Joungha Won)
- - Inverted Microscopy (Hyun ji Kang)
- - PCR machine (Moonsun Sa)
- - Electrophoresis gel runner (Hyun ji Kang)
- - Electrophoresis gel doc (Moonsun Sa)
- - Nanodrop (Joungha Won)
- - Vibratome, slice patch puller and cell patch puller (Jea Kwon, Woojin Won, Min-woo Jang)
- - Cryosection (Heeyoung An)
- - Virus injector (Sunpil Kim)
- - Electroporator (Joungha Won)
- - Water purification system (Woojin Won)
- - Heat block (Yongmin Park)
- - 37℃ Incubator for molecular assay (Woojin Won)
5) Maintaining research consumables for experiments
- - Ordering (Mingu park)
- - Tip and tube (microtube and PCR tube) (Myungsun Park)
- - 70% Ethanol (Yongmin Park)
- - Media for cell culture and E.coli (Yongmin Park)
- - Agarose gel and agar plate
- - Stock for drug, antibody, enzyme, virus and DNA
6) Public duties
- - Genotyping
- : Tail prep (Jae kyung Kwon)
- : Result should be reported by the next weaning time
- : Each person is assigned of specific mouse lines
- - Mouse maintaining: (Jae kyung Kwon)
- : Each person should take training course on animal experimentation
- : Each person should be fully informed about housing, weaning and ear-punching of mice
- - Cell culture: primary astrocyte and neuron culture, mammalian cell lines (Myungsun Park, Yongmin Park)
- - shRNA cloning & virus production: (Sol-ji Lee)
- : Using essential experimental techniques including oligomer design, qPCR/RT-PCR, transformation, mini-prep, midi-prep, DNA sequencing, cell culture, transfection
- : Receive order (cloning, virus production) from another institute
7) Experimental techniques
- - Primary astrocyte culture (Myungsun Park, Yongmin Park)
- - Primary neuron culture (Yeonha Ju)
- - Cell-line culture (Joungha Won)
- - Calcium imaging (Sunpil Kim)
- - FRET imaging (Jea Kwon)
- - Two-photon imaging (Sunpil Kim)
- - Cultured cell patch clamp recording (Min-woo Jang)
- - Sniffer patch clamp recording (Wuhyun Koh)
- - Pressure clamp recording (Hyun ji Kang)
- - Slice patch clamp recording (Jea Kwon, Woojin Won, Joungha Won, Moonsun Sa)
- - Slice field recording (Wuhyun Koh)
- - Autopatch (Hyun ji Kang, Min-woo Jang)
- - Immunohistochemistry (Heeyoung An)
- - Behavior tests (Sunpil Kim)
- - Microdialysis (Sunpil Kim)
- - Optogenetic stimulation (Joungha Won, Woojin Won)
- - Brain infusion with micropump (Sunpil Kim)
- - Virus injection (Sunpil Kim)
- - SNP analysis (Joungha Won)
- - DNA cloning (Joungha Won)
- - RNA work (Min-woo Jang)
- - qRT-PCR (Joungha Won)
- - Western blot (Min-woo Jang, Mingu Park, Taeyung Kim)
- - DAO action assay (Wuhyun Koh)
- - MAOB, H2O2 assay (Woojin Won)
- - Confocal microscopy (Heeyoung An)
- You can find more protocols from the backup folder of our lab
- ftp://203.247.189.127:4022 (Public NAS)
8) Backing up data and presentation files (Wuhyun Koh)
- - ftp://203.247.189.126:4022 (Private NAS)
- - ftp://203.247.189.127:4022 (Public NAS)
- - Each person needs username and password for access. (Password should be changed every 3 months)
- - All raw data, analysis results, and presentations files must be backed up in these public drives
9) Lab home page maintenance (Wuhyun Koh)
- - https://www.ibs.re.kr/glia/
- - Update your personal info
