EDUCATION

Undergraduate, 2001/3~2005/2, Biological Sciences, Korea University

Graduate, 2005/8~2015/8, Interdisciplinary Program in Neuroscience, Seoul National University

RESEARCH EXPERIENCES

2010/7~2014/06, Researcher, KIST

2014/07~2015/6, Visiting Graduate student, KIST

2016/7-present, Post doctoral fellow, KIST

EXPERICENED RESEARCH SKILLS

Slice Patch (Neuron and Astrocyte), Immunohistochemistry, Confocal imaging, Behavioral Test (passive avoidance test, open field test, fear conditioning, elevated plus maze), Molecular Biological Techniques (Cloning, Subcloning and RT-PCR), Stereotaxic Surgery, Cell Culture (Primary astrocyte, Primary neuron, and Cell line)

PUBLICATIONS

1. Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors. Park H, Han KS, Seo J, Lee J, Dravid SM, Woo J, Chun H, Cho S, Bae J, An H, Koh W, Yoon BE, Berlinguer-Palmini R, Mannaioni G, Traynelis SF, Bae Y, Choi SY, Lee C. Mol Brain. 2015 Feb 3;8(1):7 2. Glial GABA, synthesized by monoamine oxidase B, mediates tonic inhibition Bo-Eun Yoon, JunsungWoo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, JooOkMin, Soo-Jin Oh, Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Yong Chul Bae and C. Justin Lee. J Physiol. 2014 Sep 19. 3. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease, Seonmi Jo, Oleg Yarishkin, Yu Jin Hwang, Ye Eun Chun, Mijeong Park, Dong Ho Woo, Jin Young Bae, Taekeun Kim, Jaekwang Lee, Heejung Chun, Hyun Jung Park, Da Yong Lee, Jinpyo Hong, Hye Yun Kim, Soo-Jin Oh, Seung Ju Park, Hyo Lee, Bo-Eun Yoon, YoungSoo Kim, Yong Jeong, Insop Shim, Yong Chul Bae, Jeiwon Cho, Neil W Kowall, Hoon Ryu, Eunmi Hwang, Daesoo Kim & C Justin Lee Nat Med. 2014 Jun 29. 4. AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia. Lee YS, Choi SL, Jun H, Yim SJ, Lee JA, Kim HF, Lee SH, Shim J, Lee K, Jang DJ, Kaang BK. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15520-5. Epub 2012 Sep 4. 5. PKA-activated ApAF-ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation. Lee JA, Lee SH, Lee C, Chang DJ, Lee Y, Kim H, Cheang YH, Ko HG, Lee YS, Jun H, Bartsch D, Kandel ER, Kaang BK. J Cell Biol. 2006 Sep 11;174(6):827-38. 6. Regulation of ApC/EBP mRNA by the Aplysia AU-rich element-binding protein, ApELAV, and its effects on 5-hydroxytryptamine-induced long-term facilitation. Yim SJ, Lee YS, Lee JA, Chang DJ, Han JH, Kim H, Park H, Jun H, Kim VN, Kaang BK. J Neurochem. 2006 Jul;98(2):420-9. 7. SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses.Ko J, Kim S, Chung HS, Kim K, Han K, Kim H, Jun H, Kaang BK, Kim E. Neuron. 2006 Apr 20;50(2):233-45.

Education

2013.03 – 2017. 02 Ph.D. from College of Korean Medicine, Kyung Hee University 
Advisor: Kwang Seok Ahn, Bum Sang Shim 
Mentor: Justin C. Lee (Korea Institute of Science and Technology) 
Honors: 1st prize of oral presentation at annual workshop of Brain Science Institute, KIST (2014), Poster award at 2015 Glia Meeting Korea (2015), Poster award at Brain conference 2015 (2015), 2nd prize of oral presentation at KIST Junior R&D Expo (2016), Poster award at 2017 Glia Meeting Korea (2017)

2011.03 – 2013.02 M.S. from College of Korean Medicine, Kyung Hee University 
Advisors: Kwang Seok Ahn, Seung-Hoon Choi 
Mentor: Kwang-Sup Soh (Seoul National University) 
Thesis: Investigation of the primo vascular system underneath the superior sagittal sinus in the brain 
Honors: Korea Junior Research Fellowship (2012~2015)

2004.03 - 2011.02 MD (Korean Medicine) and B.S. from College of Korean Medicine, Kyung Hee University 
Honors: Youth Heojun Award (2010), Korea Presidential Youth Talent Award (2010), Dean's honored graduate (2011)

2001.03 - 2004.02 Korean Minjok Leadership Academy (민족사관고등학교)

Experience 
Center for glia-neuron interaction, Brain Science Institute, Korea Institute of Science and Technology 
Post-doc, 2017.03 – present

Lab for glia-neuron interaction, Center for Neural Science and Center for Functional Connectomics Center, Korea Institute of Science and Technology 
Visiting graduate student, 2013.03 – 2017.02

Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University 
Visiting graduate student, 2011.03 – 2013.02

Department of Pathology, College of Korean Medicine, Kyung Hee University 
Research assistant, 2011.03 - 2012.03

Biomedical Physics Laboratory, College of Physics & Astronomy, Seoul National University 
Researcher, 2011.01 - 2011.02

Publications – papers

Lee SW*, Nam MH*, Lee BC, Herbal Acupuncture for Type 2 Diabetes: A Systematic Review, Experimental and Therapeutic Mededicine, 2017, accepted

Nam MH, Jung SJ, Lim J, Seo E, Lim J, Lee SJ, Yoon SZ, Soh KS, A novel fibrous duct structure in the brain meninges discovered using polarized light microscopy, Journal of Korean Physical Studies, 2016;68(10):1246-53.

Nam MH*, Lee SW*, Na HY, Yoo JH, Paik SH, Ahn KS, Ahn YM, Ahn SY, Choi SH, Lee BC, Herbal Acupuncture for the Treatment of Obesity, Journal of Acupuncture & Meridian Studies, 2016, 9(2):49-57.

Kim J, Stefanov M, Nam MH, Kim S, Tracing Mercox Injected at Acupuncture Points under the Protocol of Partial Body Macerations in Mice, Journal of Acupuncture & Meridian Studies, 2015;8(6):314-20.

Nam MH, Ahn KS, Choi SH. Acupuncture: A potent tool for inducing adult neurogenesis, Neural Regeneration Research, 2015;10(1):33-35.

Nam MH*, Baek M*, Lim J, Lee S, Yoon J, Kim J, Lee MS, Soh KS. Discovery of a novel fibrous tissue in the spinal pia mater by polarized light microscopy, Connective Tissue Research, 2014;55(2):147–155.

M. Stefanov, M. Potroz, J. Kim, J Lim, Richard Cha, MH Nam. The Primo Vascular System as a New Anatomical System, Journal of Acupuncture & Meridian Studies, 2013;6(6):331-338.

SJ Jung, KH Bae, MH Nam, HM Kwon, YK Song, KS Soh, Primo Vascular System Floating in Lymph Ducts of Rats, Journal of Acupuncture & Meridian Studies, 2013;6(6):306-318. (Featured article)

Nam MH, Ahn KS, Choi SH. Acupuncture stimulation induces neurogenesis in adult brain, International Review of Neurobiology, 2013;111:67-90.

Lee SH, Bae KH, Kim GO, Nam MH, Choi YB, Kwon HM, Ryu Y, Soh KS. Primo vascular system in the lymph vessel from the inguinal to the axillary nodes, Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 472704, 6 pages, 2013. doi:10.1155/2013/472704.

Bae KH, Park SH, Lee BC, Nam MH, Yoon JW, Kwon HM, Yoon SZ. Novel threadlike structures may be present on the large animal organ surface: evidence in swine model, Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 758763, 5 pages, 2013. doi:10.1155/2013/758763.

Stefanov M, Kim JD, Nam MH, Soh KS. New approach of corrosion casting using direct injection of mercox into the parenchyma of different organs, The Anatomical Record, 2013;296:724–725.

Nam MH, Lim J, Choi SH, Kim SC, Soh KS. A primo vascular system underneath the superior sagittal sinus in the brain of a rabbit, Journal of Acupuncture & Meridian Studies, 2012;5(5):210-217.

Nam MH, Choi SH, Soh KS. Light and primo vascular system in the brain, Journal of the Korean Physical Society, 2012;60(6):903-906.

Nam MH, Yin CS, Choi SH, Soh KS. Adult neurogenesis and acupuncture stimulation at ST36, Journal of Acupuncture & Meridian Studies, 2011;4(3):153-158.

Nam MH, Kim Y, Kim H, Park J, Yeh HY, Lee YS, Lee S, Jung WM, Hong YK, Lee H, Park HJ, Chae Y, A Comparison Study of Factors of Willingness to Use Traditional Medicine between Korean, Chinese and Taiwanese, J Korean Oriental Med 2011;32(1):36-55.

Kim SY, Kim H, Nam MH, Li Y, Chung HC, Park HJ, Lee H, Chae Y, Evaluation of a Traditional Korean Medicine Content Factor and Satisfaction with the Drama ‘Daejanggeum’, The Journal of Korean Acupuncture & Moxibustion Society, 2010;27(1):11-20.

Wuhyun Koh

Birth Date : November 12, 1989

Citizenship : Republic of Korea

Tel : +82-10-6361-2748

Email : whvankoh@gmail.com (general) or whvankoh@ibs.re.kr (institute)

Current Title : Ph. D. course

EDUCATION

2008.03 ~ 2012.08

B.A. in Korea University, Republic of Korea

2013.08 ~ 2018.11

Researcher, Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Republic of Korea

2013.08 ~ present

Integrated Ph.D. course, in University of Science and Technology (UST), Republic of Korea

2018.02 ~ present

Technical Research Personnel (Mandatory military service in Republic of Korea)

2018.12 ~ present

Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS), Republic of Korea

RESEARCH INTERESTS

Astrocyte-Neuron Interaction, Reversal Learning

PUBLICATIONS

S.-J. Oh, J. Lee, H.-B. Kim, J. Lee, S. Han, J. Bae, G.-S. Hong, W. Koh, J. Kwon, E.-S. Hwang, D. Woo, I. Youn, I.-J. Cho, Y. Bae, S. Lee, J. Shim, J.-H. Park, J. C. Lee, Ultrasonic Neuromodulation via Astrocytic TRPA1. Current Biology 29, 3386-797583616 (2019)10.1016/j.cub.2019.08.021).

M.-H. Nam, K.-S. Han, J. Lee, W. Won, W. Koh, J. Bae, J. Woo, J. Kim, E. Kwong, T.-Y. Choi, H. Chun, S. Lee, S.-B. Kim, K. Park, S.-Y. Choi, Y. Bae, J. C. Lee, Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference. Cell Reports 28, 1154-116600000 (2019)10.1016/j.celrep.2019.06.071).

J. Woo, Y.-E. Han, W. Koh, J. Won, M. Park, H. An, J. C. Lee, Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient. Experimental Neurobiology 28, 30-42 (2019)10.5607/en.2019.28.1.30).

W. Koh, Y. Park, S. Lee, J. C. Lee, AAV-Mediated Astrocyte-Specific Gene Expression under Human ALDH1L1 Promoter in Mouse Thalamus. Experimental Neurobiology 26, 350-361 (2017)10.5607/en.2017.26.6.350).

H. Park, K.-S. Han, J. Seo, J. Lee, S. M. Dravid, J. Woo, H. Chun, S. Cho, J. Bae, H. An, W. Koh, B.-E. Yoon, R. Berlinguer-Palmini, G. Mannaioni, S. F. Traynelis, Y. Bae, S.-Y. Choi, J. C. Lee, Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors. Molecular Brain 8, 1-16 (2015)10.1186/s13041-015-0097-y).

EXPERIENCES

2009. 06 ~ 2009. 08 48th National Undergraduate Symposium on Biology (NUSB),
Topic : Mitochondia

2010. 06 ~ 2010. 08 49th National Undergraduate Symposium on Biology (NUSB),
Topic : Epigenetics

2010. 06 ~ 2011. 03 Undergraduate Internship Program in Lab of Neuroscience (Dr. Bongjune Yoon), Korea University, Republic of Korea

2011. 06 SURF (Summer Undergraduate Research Fellowship) program in Molecular Neuroscience Lab (Dr. JungHoon Kim), POSTECH, Republic of Korea

2011. 02 ~ 2011. 08 50th National Undergraduate Symposium on Biology (NUSB) Staff,
Topic : Neuroscience

2012. 06 ~ 2012. 08 51st National Undergraduate Symposium on Biology (NUSB),
Topic : Bioinformatics

2013. 04 ~ 2013. 08 Undergraduate Internship Program in Glia-Neuron Interaction Lab
(Dr. C. Justin Lee), KIST, Republic of Korea

AWARDS

1st prize for “Best oral presentation” at the 2018 Brain Science Institute (BSI) annual meeting held in

Pyeongchang, Republic of Korea

Personal Record

Name                                       : Joungha Won

Country of Citizenship            : Republic of Korea

Current Status                     : Post-Doctoral fellow, Center for Cognition and Sociality, Institute for Basic Science (IBS)

E-mail                                      : jwon@ibs.re.kr

Education

2007. 03 ~ 2013. 02               BS, Department of Biology, Kyung Hee University

2013. 03 ~ 2021. 02               Integrated MS/Ph.D, program, Department of Biological Science, KAIST

Academic Appointments

2013. 02 ~                                Ph.D. Candidate, Bio-Imaging and Optogenetics Laboratory, KAIST 
                 (Professor Won Do Heo’s Lab)

2015. 03 ~ 2018. 10                Student Researcher, Center for Neuroscience,

Center for Glia-Neuron interaction, KIST (C. Justin Lee’s Lab)

2018.11 ~                                Student Researcher, Center for Cognition and Sociality,

                                                Cognitive Glioscience Group, IBS (C. Justin Lee’s lab)

Thesis title

Development of optogenetic molecular probe for inhibition of synaptic transmission, and detection protocol for the COVID-19

Research Interests

Activity-dependent astrocytic proBDNF release in Learning and Memory

Activity-dependent and environment-dependent BDNF expression in astrocyte.

Development an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission and behavior

Investigate calcium activity and its functions in astrocyte.

The function of astrocytic molecule in pathophysiological status, especially in stroke and Parkinson's disease.

Diagnostic tool development.

Experimental Techniques

Imaging experiment: Confocal imaging, Live-cell imaging, Intrinsic optical signaling imaging, Super-resolution imaging, Calcium imaging, Total internal reflection fluorescence imaging, multi-photon image, etc.

Electrophysiology: whole-cell patch clamp in acute brain tissue and cultured cell, and field recording

Molecular biology: SNP analysis, cloning, qRT-PCR, diagnosis tool development, etc.

Next-Generation Sequencing (NGS)

Primary culture: primary cortical, hippocampal and spinal cord astrocyte cell culture, cortical neuron culture

Animal behavior experiment: Fear conditioning, Open field test, Novel object/place recognition test, Passive avoidance test, etc.

Histology: Immunohistochemistry, Immunocytochemistry

Optogenetics experiment: Imaging, molecular biology, and behavior experiment with optogenetic tools.

Awards

Best presentation Award, The Korean Society for Brain and Neural Sciences, 2020

Best article award of The Korean Society for Brain and Neural Sciences, The Korean Society for Brain and Neural Sciences, 2021

Best presentation Award, The Korean Society for Brain and Neural Sciences, 2021

Publications

Published

1. Joungha Won, Lee S, Park M, Kim TY, Park GM, Choi BY, Kim D, H Chnag, Heo WD, Kim V. N and Lee CJ., Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)., Experimental Neurobiology Exp Neurobiol. 2020 Apr;29(2), 11 March (2020)

2. Joungha Won, Hasan Hüseyin Kazan, Jea Kwon, Myungsun Park, Mehmet Ali Ergun, Sureyya Ozcan, Byung Yoon Choi, Won Do Heo^* and C. Justin Lee^*, Ultimate COVID-19 Detection Protocol Based on Saliva Sampling and qRT-PCR with Risk Probability Assessment, Experimental Neurobiology Exp Neurobiol. 2021 Feb 28; 30(1): 13–31.

3. Chang KY, Woo D, Jung H, Lee S, Kim S, Won Joungha, Kyung T, Park H, Kim N, Yang HW, Park JY, Hwang EM, Kim D, Heo WD., Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling., Nature Communications. 2014 Jun 4;5:4057. doi: 10.1038/ncomms5057.

4. Kwon J, An H, Sa M, Joungha Won, Shin JI, Lee CJ., Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocyte., Exp Neurobiol 2017 Feb;26(1):42-54. (2017)

5. Woo DH, Bae JY, Nam MH, An H, Ju YH, Joungha Won, Choi JH, Hwang EM, Han KS, Bae YC, Lee CJ, Activation of Astrocytic μ-opioid Receptor Elicits Fast Glutamate Release Through TREK-1-Containing K2P Channel in Hippocampal Astrocytes., Front Cell Neurosci. 2018 Sep 27;12:319. (2018)

6. Woo J, Han YE, Koh W, Joungha Won, Park MG, An H, Lee CJ., Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient., Exp Neurobiol. 2019 Feb;28(1):30-42 (2019)

7. Han YE, Kwon J, Joungha Won, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ., Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain., Exp Neurobiol. 2019 Apr;28(2):183-215 (2019)

8. Park M, Joungha Won, Chio BY, Lee CJ., Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR., Experimental & Molecular Medicine 52, pages963–977(2020)

9. Kim TY, Jeon S, Jang Y, Gotina Lizaveta, Joungha Won, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim S, Lee CJ, Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome-and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion, Experimental & Molecular Medicine, 2021

Under Review/Accepted article (on 7^th, Jan., 2021)

1. Joungha Won, Pankratov Y, Lee S, Kim A, Jang MW, Lee CJ*., Heo WD*., Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission and behavior., Neuron, Under review

1. EDUCATION:

- B.S. 2002/2 Biotechnology

Department of Biotechnology, Yonsei University, Seoul, Korea

- M.S. 2004/2 Microbialgenetics

School of Biological Sciences, SeoulNationalUniversity,Seoul,Korea

Dissertation Title: “Genetic Engineering to Improve the Zymomonas mobilis ZM4”

Advisor: Hyen Sam Kang, Ph.D.

- Ph.D. 2014/2 Neuroscience

Department of Craniomaxillofacial Cell and Developmental Biology

School of Dentistry,SeoulNationalUniversity,Seoul,Korea

Dissertation Title: “Expression, Function and Regulation of Bestrophin-1 Channel in Astrocyte”

Advisor: Seungbok Lee, Ph.D. and C. Justin Lee, Ph.D.

2. RESEARCH EXPERIENCE:

Jun. 2000 ~ Feb. 2001 Research assistant in Biochemistry and virus laboratory (Advisor: Baik-Lin Seong, Ph.D. professor in Yonsei University)

Sep. 2002 ~ Jun. 2003 Teaching assistant in School of Biological Sciences, Seoul National University

Jan. 2004 ~ Mar. 2005 Research Scientist in Biomedical Center, KIST (Korea Institute of Science and Technology. Advisor: Yeon Gyu Yu, Ph.D. Principal Research Scientist in KIST)

Apr. 2005 ~ Feb. 2016 Research Scientist in Center for Neuroscience, KIST (Korea Institute of Science and Technology. Advisor: C. Justin Lee, Ph.D. Principal Research Scientist in KIST)

Jun. 2012 ~ Jul. 2012 Visiting researcher in The Marine Biological Laboratory (MBL) in Woods Hole

Mar. 2016 ~ Present Senior Research Scientist in Convergence Research Center for Diagnosis, Treatment and Care System of Dementia and Center for Glia-Neuron interaction, KIST (Korea Institute of Science and Technology)

3. PUBLICATIONS :

1. Jeong-Sun Seo, Hyonyong Chong, Hyun Seok Park, Kyoung-Oh Yoon, Cholhee Jung, Jae Joon Kim, Jin Han Hong, Hyungtae Kim, Jeong-Hyun Kim, Joon-Il Kil, Cheol Ju Park, Hyun-Myung Oh, Jung-Soon Lee, Su-Jung Jin, Hye-Won Um, Hee-Jong Lee, Soo-JinOh, Jae Young Kim, Hyung Lyun Kang, Se Yong Lee, Kye Joon Lee and Hyen Sam Kang. “The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4” Nature Biotechnology (2004) 23: 63-68

2. Soo-JinOh, JungHwanPark,SungyuHan,JaeKyunLee,EunJooRohandCJustinLee.“DevelopmentofselectiveblockersforCa2+-activatedCl-channelusingXenopus laevis oocytes with an improved drug screening strategy” Molecular Brain (2008) 1: 14-24 [first author]

3. Yun-Kyoung Kim, Soo-JinOh, Bong-Suk Jin, ChanhooPark,HyesungJeon,DooWanBoo,andYeonGyuYu.“Acell-basedmethodtomonitortheinteractionbetweenhepatitisBviruscapsidandsurfaceproteins”Bulletin of the Korean Chemical Society (2009) 30: 577-581

4. Mihye Lee, Sang Kyoo Paik, Min-Jung Lee, Yoon-Jung Kim, Sungdae Kim, Minyeop Nahm, Soo-JinOh, Hyun-Man Kim, Jeongbin Yim, C. Justin Lee, Yong Chul Bae and Seungbok Lee. “Drosophila atlastin regulates the stability of muscle microtubules and is required for synapse development” Developmental Biology (2009) 330: 250-262

5. HyungjuPark*,Soo-JinOh*, Kyung-Seok Han, Dong Ho Woo, HyekyungPark,GuidoMannaioni,StephenF.Traynelis,andC.JustinLee.“Bestrophin-1encodesfortheCa2+-activatedanionchannelinhippocampalastrocytes” The Journal of Neuroscience (2009) 29: 13063-13073 [*co-first authors]

6. Soojung Lee, Bo-Eun Yoon, Ken Berglund, Soo-JinOh, HyungjuPark,Hee-SupShin,GeorgeJ.Augustine,C.JustinLee.“Channel-mediatedtonicGABAreleasefromglia”Science (2010) 330: 790-796

7. Rae Hyung Ryu, Soo-JinOh, Ra Mi Lee, Seong Won Jeong, Lily Yeh Jan, Chi Ho Lee, C. Justin Lee, Sang Min Jeong. “Cloning and heterologous expression of new xANO2 from Xenopus laevis” Biochemical and Biophysical Research Communications (2011) 408: 559-565

8. Ra Mi Lee, Rae Hyung Ryu, Seong Won Jeong, Soo-JinOh, Hue Huang, Jin Soo Han, Chi Ho Lee, C. Justin Lee, Lily Yeh Jan, and Sang Min Jeong. “Isolation and Expression Profile of the Ca2+-ActivatedChlorideChannel-likeMembraneProtein6GeneinXenopus laevis” Laboratory Animal Research (2011) 27: 109-116

9. Dong Ho Woo, Kyung-Seok Han, Jae Wan Shim, Bo-Eun Yoon, Eunju Kim, Jin Young Bae, Soo-JinOh, Eun Mi Hwang, Alan D. Marmorstein, Yong Chul Bae, Jae-Yong Park, and C. Justin Lee. “TREK-1 and Best1 Channels Mediate Fast and Slow Glutamate Release in Astrocytes upon GPCR Activation” Cell (2012) 151(1): 25-40

10. Soo-Jin Oh, Kyung-Seok Han, Hyungju Park, Dong Ho Woo, Hye Yun Kim, Stephen F. Traynelis, and C Justin Lee. “Protease Activated Receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis” Molecular Brain (2012) 5:38 [first author]

11. Soo-JinOh, Seok Jin Hwang, Jonghoon Jung, Kuai Yu, Jeongyeon Kim, Jung Yoon Choi, H.Criss Hartzell, Eun Joo Roh, and C. Justin Lee. “MONNA, a Potent and Selective Blocker for Transmembrane Protein with Unknown Function 16/Anoctamin-1” Molecular Pharmacology (2013) 84:726-735 [first author]

12. Hyungju Park, Kyung-Seok Han, Soo-JinOh, Seonmi Jo, Junsung Woo, Bo-Eun Yoon, and C. Justin Lee. “High glutamate permeability and distal localization of Best1 channel in CA1 hippocampal astrocyte” Molecular Brain (2013) 6:54

13. Seonmi Jo, Oleg Yarishkin, Yu Jin Hwang, Ye Eun Chun, Mijeong Park, Dong Ho Woo, Jin Young Bae, Taekeun Kim, Jaekwang Lee, Heejung Chun, Hyun Jung Park, Da Yong Lee, Jinpyo Hong, Hye Yun Kim, Soo-JinOh, Seung Ju Park, Hyo Lee, Bo-Eun Yoon, YoungSoo Kim, Yong Jeong, Insop Shim, Yong Chul Bae, Jeiwon Cho, Neil W Kowall, Hoon Ryu, Eunmi Hwang, Daesoo Kim and C Justin Lee. “GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease” Nature Medicine (2014) doi:10.1038/nm.3639

14. Seung Eun Lee, Jaekwang Lee, Charles Latchoumane, Boyoung Lee, Soo-JinOh, Zahangir Alam Saud, Cheongdahm Park, Ning Sun, Eunji Cheong, Chien-Chang Chen, Eui-Ju Choi, C. Justin Lee, and Hee-Sup Shin. “Rebound burst firing in the reticular thalamus is not essential for pharmacological absence seizures in mice” PNAS (2014) 111(32): 11828-11833

15. Bo-Eun Yoon, Junsung Woo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, Joo Ok Min, Soo-JinOh, Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Young Chul Bae, and C. Justin Lee. “Glial GABA, synthesized by monoamine oxidase B, mediates tonic inhibition” The Journal of Physiology (2014) Sep 19

4. PATENTS :

1. Use of anthranillic acid derivatives on calcium activated chloride channel (#10-0892591 02-Apr-2009)

2. Cellular imaging of the interaction between HBV capsid and surface proteins, and its application for screening of inhibitory ag (#10-0949310 17-Mar-2010)

3. Method for screening a glutamate release inhibitor in an astrocyte (#8,871,463 28-Oct-2014, # 10-1418061 3-Jul-2014)

4. Novel compounds as chloride channel blocking agent (#10-1464280 17-Nov-2014)

5. AWARDS and HONORS :

Brain Korea 21 (BK21) Scholarship (2002 - 2003)

KIST우수연구개발팀상 (2011)

Poster award in 2014 BSI symposium in Korea

Poster award in 2016 The Glia Section of the Korea Society of Brain and Neural Science

Travel award in Japan Neuroscience Society 2016

6. POSTER Presentation :

1. The 12th Japan-Korea Joint symposium on Drug Design and Development Jun-2008

Title: Synthesis and biological activities of anthranilic acid derivatives as Ca2+activatedCl-channelblockers

2. International Society for Neurochemistry Aug-2009

Title: Bestrophin-1 Encodes for the Ca2+ActivatedAnionChannelinhippocampalastrocytes

3. Society For Neuroscience Nov-2010

Title: Astrocytic Ca2+-activatedanionchannel,Bestrophin-1,ispermeabletoglutamateandGABA

4. Society For Neuroscience Nov-2013

Title: MONNA, a potent and selective blocker for TMEM16A/Anoctamin-1

5. The Glia Section of the Korea Society of Brain and Neural Science

Title: Ultrasonic brain stimulation modulates neuronal activity via mechano-sensitive TRPA1 channels in astrocytes Jan-2016

Education

March 2011 – February 2015, Konkuk University, Major: Life science

March 2015 – Present, University of Science & Technology (UST) in Korea, Major: Neuroscience

Work experience

February 2014 – March 2014, Internship program in Korean basic science institute (KBSI), study for two photon microscope confocal microscope with IHC

March 2014 – February 2015, Internship in Korea Institute of science and technology (KIST)

Experienced research skills

Slice Patch, Immunohistochemistry, Confocal imaging, Behavior test (Passive avoidance test, forced swimming test, Rotarod test), Molecular biological techniques (Cloning, Subcloning and RT-PCR, shRNA making), Stereotaxic Surgery, Cell culture (Primary astrocyte, Primary neuron, cell lines)

Publication

Dong Ho Woo,† Jin Young Bae,† Min-Ho Nam,† Heeyoung An, Yeon Ha Ju, Joungha Won, Jae Hyouk Choi, Eun Mi Hwang, Kyung-Seok Han, Yong Chul Bae, and C. Justin Lee (2018). "Activation of Astrocytic mu-opioid Receptor Elicits Fast Glutamate Release Through TREK-1-Containing K2P Channel in Hippocampal Astrocytes." Frontiers in Cellular Neuroscience 12.

Jong-Hyun Park†, Yeon Ha Ju†, Ji Won Choi, Hyo Jung Song, Bo Ko Jang, Junsung Woo, Heejung Chun, Hyeon Jeong Kim, Su Jeong Shin, Oleg Yarishkin, Seonmi Jo, Mijeong Park, Seul Ki Yeon, Siwon Kim, Jeongyeon Kim, Min-Ho Nam, Ashwini M. Londhe, Jina Kim, Sung Jin Cho, Suengmok Cho, Changho Lee, Sung Yeoun Hwang, Sang Wook Kim, Soo-Jin Oh, Jeiwon Cho, Ae Nim Pae, C. Justin Lee, Ki Duk Park (2019). “Newly developed reversible MAO-B inhibitor circumvents the shortcomings of irreversible inhibitors in Alzheimer’s disease.” Science Advance 5.

Awards

Oral presentation in KIST - BSI conference (Sep, 2017) _New reversible MAO-B inhibitor KDS2010 abolishes astrogliosis and memory impairment in Alzheimer’s disease model

Poster presentation at Korea Society of Neurodegeneration Disorder (KSND) (Mar, 2018) _ PyrPeg, a Novel Two-photon Probe to Selectively Detect Aβ in Alzheimer’s Disease

undergraduate : 2009.03~2016.02 Korea University

Contact details

Name: Myung sun Park

Date of birth and Place: February 19. 1994, Seoul, Korea

Nationality: Republic of Korea

Home address: 305, 118-28, Sinseong-dong, Yuseong-gu, Daejeon City, South Korea

Tel) 82-10-8949-2583 e-mail) bak100000s@gmail.com

Education and Training

M.S., Hannam University, Daejeon, Republic of Korea, 2016-2018

Department of Biological Science and Biotechnology

B.S., Hannam University, Daejeon, Republic of Korea, 2012-2016

Department of Biological Science and Biotechnology

Dissertation Titles

Abnormality of slow fiber in skeletal muscle of Huntington's disease mouse model (MS).

Professional Experiences

Researcher, Institute for Basic Science, Daegu, Republic of Korea, Daejeon, Republic of Korea, 2018-present

Cognition and Sociality Center

Researcher, Kyungpook National University, Daegu, Republic of Korea, 2018

Medical Research Center

Researcher, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, Republic of Korea, 2016-2018

Aging Research Center

 Publications

 1. Park, M., Min, B., Jeon, K., Cho, S., Park, J. S., Kim, J., . . . Kang, Y. K. (2017). Age-associated chromatin relaxation is enhanced in Huntington's disease mice. Aging (Albany NY), 9(3), 803-822. doi:10.18632/aging.101193

 2. Min, B*., Park, M*., Jeon, K., Park, J. S., Seo, H., Jeong, S., & Kang, Y. K. (2018). Age-associated bimodal transcriptional drift reduces intergenic disparities in transcription. Aging (Albany NY), 10(4), 789-807. doi:10.18632/aging.101428, *co-first author.

Research Presentations

1. “Age-dependent reduction transcriptional disparities”, “Global hypomethylation on large retroelements in aged muscle” Spring Conference of the Korean Society for Gerontology, 2018, poster presentation

2. "Age-associated chromatin relaxation is enhanced in Huntington's disease mice", KSMCB에피유전체학분과 심포지움, 2017, poster presentation

Tai Young Kim

• Senior Researcher

• Cholesteol metablisim in glioblastoma, Indentification of interacting proteins using BioID, Drug development against COVID-19 coronavirus (SARS-CoV-2)

• taik@ibs.re.kr
  

EDUCATION

2002             Ph.D. in Tumor Biology, Seoul National University, College of Medicine, Korea (advisor: Yung-Jue Bang)

1996             M.S. in Microbiology, Seoul National University, Korea (advisor: Gajin Jeong)

1993             B.S. in Microbiology, Hankuk University of Foreign Studies, Korea.

PROFESSIONAL EXPERIENCES

06/2020 – Current    Senior Researcher, Center for Cognitive and Sociality, Institute for Basic Science, Daejeon, Korea

01/2019 – 05/2020   Research Fellow, Cognitive Glioscience Group, Center for Cognitive and Sociality, Institute for Basic Science, Daejeon,

                            Korea

11/2015 – 12/2018   Research Professor, Kyung Hee University Korean Medicine, Korea.

01/2014 – 10/2015   Principal Research Scientist, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National

                            University, Korea.

08/2013 – 12/2013   Research Associate Professor, School of Nano-Bioscience and Chemical Engineering, UNIST, Republic of Korea

01/2009 - 07/2013   Postdoctoral Research Associate, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC

                            (Advisor: Channing J. Der)

01/2004 - 12/2008   Postdoctoral Research Associate, Department of Pharmacology, University of North Carolina, Chapel Hill, NC

                            (Advisor:  Rudy L. Juliano)

08/2002 - 12/2003   Postdoctoral Research Associate, Cancer Research Institute, Seoul National University, Korea (advisor: Yung-Jue Bang)

07/1998 - 03/1999   M.S. Researcher, Cancer Research Institute, Seoul National University, Korea

AWARDS and FELLOWSHIPS

2016-2019    Research Fellow Grant, Ministry of Education, Korea of Republic

2013–2016    Research Fellow Grant, Ministry of Education, Korea of Republic

2009-2011    Susan G. Komen Research Grant

2003            Young Researcher Awards in Seoul National University Cancer Research Institute

PUBLICATION

1. Jang MW, Oh D, Yi E, Liu X, Ling J, Kim N, Sharma K, Kim TY, Lee S, Kim AR, Kim MY, Kim MA, Lee M, Han JH, Han JJ, Park HR, Kim BJ, Lee SY, Woo DH, Oh J, Oh SJ, Du T, Koo JW, Oh SH, Shin HW, Seong MW, Lee KY, Kim UK, Shin JB, Sang S, Cai X, Mei L, He C, Blanton SH, Chen ZY, Chen H, Liu X, Nourbakhsh A, Huang Z, Kang KW, Park WY, Feng Y, Lee CJ, Choi BY (2021) A nonsense TMEM43 variant leads to disruption of connexin-linked function and autosomal dominant auditory neuropathy spectrum disorder. Proc Natl Acad Sci, USA. Jun 1;118(22)

2. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim ST, Lee CJ (2021) Platycodin D, a natural product from Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med, May;53(5):956-972

3. Lee SJ, Choi YJ, Kim HI, Moon HE, Baek SH, Kim TY, Ko SG (2021) LDLR facilitates platycodin D-induced autophagy inhibition.and cell death in glioblastoma cells. Mol Oncol. May 1 *Co-corresponding Author.

4. Kim HI, Lee SJ, Choi YJ, Kim MJ, Kim TY, Ko SG (2021) Quercetin induces apoptosis in glioblastoma cell lines U87MG and U373MG by suppressing Axl/IL-6/STAT3 signaling pathway. Am J Chin Med, Mar 3;1-18. *Co-corresponding Author

5. Choi YJ, Lee SJ, Kim HI, Lee HJ, Kang SJ, Kim TY, Cheon C, Ko SG. (2020) Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells. Sci Rep, Nov 16;10(1):1983. *Co-corresponding Author

6. Won J, Lee S, Park M, Kim TY, Park MG, Choi BY, Kim D, Chang H, Kim VN, Lee CJ (2020) Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19). Exp Neurobiol, Apr 30; 29(2):107-119.

7. Hong SH, Ku JM, Kim HI, Kim TY, Seo HS, Shin YC, Ko SG (2019) Topical Application of KAJD Attenuates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms Through Regulation of IgE and MAPK Pathways in BALB/C Mice and Several Immune Cell Types. Front Pharmacol. Sep 19; 10:1097.

8. Kim HI, Hong SH, Ku JM, Lim YS, Lee SJ, Song J, Kim TY, Cheon C, Ko SG. (2019) Scutellaria Radix Promotes Apoptosis in Non-Small Cell Lung Cancer Cells via Induction of AMPK-Dependent Autophagy. Am J Chin Med. 47(3):691-705

9. Ku JM, Hong SH, Kim SR, Choi HS, Kim HI, Kim DU, Oh SM, Seo HS, Kim TY, c, Cheon C, Ko SG. (2018) Novel mechanism The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo. BMC Complement Altern Med. Jul 13;18(1):215

10. Kim HI, Hong SH, Ku JM, Kang S, Kim TY, Shin YC, Ko SG. (2017) Tonggyu-tang, a traditional Korean medicine, suppresses pro-inflammatory cytokine production through inhibition of MAPK and NF-κB activation in human mast cells and keratinocytes. BMC Complement Altern Med. Mar 31;17(1):186

11. Kim HJ, Kwon S, Nam SH, Jung JW, Kang M, Ryu J, Kim JE, Cheong JG, Cho CY, Kim S, Song DG, Kim YN, Kim TY, Jung MK, Lee KM, Pack CG, Lee JW. (2017) Dynamic and coordinated single-molecular interactions at TM4SF5-enriched microdomains guide invasive behaviors in 2- and 3-dimensional environments.FASEB J. 2017, 31(4):1461-1481.

12. Choi J, Kang M, Nam SH, Lee GH, Kim HJ, Ryu J, Cheong JG, Jung JW, Kim TY, Lee HY, Lee JW (2015) Bidirectional signaling between TM4SF5 and IGF1R promotes resistance to EGFR kinase inhibitors. Lung Cancer, 90(1):22-31

13. Nam SH, Kim D, Lee MS, Lee D, Kwak TK, Kang MK, Ryu J, Kim HJ, Song HE, Choi J, Lee GH, Kim SY, Park SH, Kim DG, Kwon NH, Kim TY, Thiery JP, Kim S, and Lee JW (2015) Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels. Oncotarget, 6(25): 21655-74

14. Lee D, Na J, Ryu J, Kim HJ, Nam SH, Kang M, Jung JW, Lee MS, Song HE, Choi J, Lee GH, Kim TY, Chung JK, Park KH, Kim SH, Kim H, Seo H, Kim P, Youn H, Lee JW (2015) Interaction of tetraspan(in) TM4SF5 with CD44 promotes self-renewal and circulating capacities of hepatocarcinoma cells. Hepatology, 61(6):1978-97

15. Kim TY, Seisser PM, Rossman KL, Goldfarb D, Mackinnon K, Yan F, Yi X, MacCoss, M, Moon RT, Der CJ, Major MB (2015) Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase. Mol Cell Biol, 35(1):167-81

16. Kang M, Ryu J, Lee D, Lee MS, Kim HJ, Nam SH, Song HE, Choi J, Lee GH, Kim TY, Lee H, Kim SJ, Ye SK, Kim S, Lee JW (2014) Correlations between Transmembrane 4 L6 Family Member 5 (TM4SF5), CD151, and CD63 in Liver Fibrotic Phenotypes and Hepatic Migration and Invasive Capacities. PLoS One. 9(7):e102817

17. Lee MS, Kim S, Kim BG, Won C, Nam SH, Kang S, Kim HJ, Kang M, Ryu J, Song HE, Lee D, Ye SK, Jeon NL, Kim TY, Cho NH, Lee JW (2014) Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation. Biochim Biophys Acta. 1843(9):2037-54

18. Kim TY, Jackson S, Xiong Y, Whitsett TG, Lobello JR, Tran NL, Malek NP, Bang YJ, Der CJ (2013) Loss of DLC1 RhoGAP tumor suppressor expression and function by CUL4A-DDB1-FBXW5-dependent ubiquitination and degradation in non-small cell lung cancer cells. Proc Natl Acad Sci, USA. 110(42):16868-73 

19. Vigil D, Kim TY, Plachco A, Garton AJ, Castaldo L, Pachter JA, Dong H, Chen X, Tokar B, Campbell SL, Der CJ (2012) ROCK1 and ROCK2 are required for Non-Small Cell Lung Cancer Anchorage-Independent Growth and Invasion. Cancer Res. 72(20):5338-47

20. Kim TY, Vigil D, Der CJ, Juliano RL (2009) Role of DLC-1, a Tumor Suppressor Protein with RhoGAP activity, in Regulation of the Cytoskeleton and Cell Motility. Cancer Metastasis Reviews28(1-2):77-83.

21. Kim TY, Kim IS, Jong HS, Lee JW, Kim TY, Jung M, and Bang YJ. (2008) Transcriptional Induction of DLC-1 gene through Sp1 sites by histone deacetylase inhibitors in gastric cancer cells. Exp Mol Med 40 (6):639-46.

22. Kim TY, Healy KD, Der CJ, Sciaky N, Bang YJ, Juliano RL (2008) Effects of Structure of Rho GTPase-activating Protein DLC-1 on Cell Morphology and Migration. J Biol Chem 283(47): 32762-70.

23. SchrampM, Ying O, Kim TY, Martin GS. (2008) ERK5 Promotes Src-induced Podosome Formation by limiting Rho activation. J Cell Biol. 181(7):1195-210.

24. Healy KD, Hodgson L, Kim TY, Shutes A, Maddileti S, Juliano RL, Hahn KM, Harden TK, Bang YJ, Der CJ. (2008) DLC-1 Suppresses Non-Small Cell Lung Cancer Growth and Invasion by RhoGAP-Dependent and Independent Mechanisms. Mol Carcinog. 47(5):326-37.

25. Jung Y, Park J, Kim TY, Park JH, Jong HS, Im SA, Robertson KD, Bang YJ, Kim TY. (2007). Potential advantages of DNA methyltransferase 1 (DNMT1)-targeted inhibition for cancer therapy. J Mol Med. 85(10):1137-48.

26. Kim TY, Lee JW, Kim HP, Jong HS, Kim TY, and Bang YJ. (2007) DLC-1, a GTPase-activating protein for Rho, is associated with cell proliferation, morphology, and migration in human hepatocellular carcinoma. Biochem Biophys Res Commun. 355(1):72-7.

27. Lee KW, Kim JH, Park JH, Kim HP, Song SH, Kim SG, Kim TY, Jong HS, Jung KH, Im SA, Kim TY, Kim NK, Bang YJ. (2006). Antitumor Activity of SK-7041, a Novel Histone Deacetylase Inhibitor, in Human Lung and Breast Cancer Cells. Anticancer Res. 26(5A):3429-38.

28. Lee SY, Kim TY, Lee MS, Kim YB, Chung E, Kim S, Lee JW. (2006). Focal adhesion and actin organization by a cross-talk of TM4SF5 with integrin alpha2 are regulated by serum treatment. Exp Cell Res. 312(16):2983-99.

29. Juliano RL, Dixit VR, Kang H, Kim TY, Miyamoto Y, Xu D. (2005). Epigenetic manipulation of gene expression: a toolkit for cell biologists. J. Cell Biol. 169(6):847-57

30. Kim JH, Lee KW, Jung Y, Kim TY, Ham HS, Jong HS, Jung KH, Im SA, Kim TY, Kim NK, Bang YJ. (2005). Cytotoxic effects of pemetrexed in gastric cancer cells. Cancer Sci. 96(6):365-71.

31. Kim HP, Kim TY, Lee MS, Jong HS, Kim TY, Lee JW, Bang YJ. (2005). TGF-1-mediated activations of c-Src and Rac1 modulate levels of cyclins and p27^Kip1 CDK inhibitor in hepatoma cells replated on fibronectin. Biochim Biophys Acta (BBA)-Mol Cell Res. 1743(1-2):151-61.

32. Yim HW, Jong HS, Kim TY, Choi HH, Kim SG, Song SH, Kim J, Ko SG, Lee JW, Kim TY, Bang YJ. (2005). Cyclooxygenase-2 Inhibits Novel Ginseng Metabolite-Mediated Apoptosis. Cancer Res. 65(5):1952-60.

33. Kim TY, Jong HS, Jung Y, Kim TY, Kang GH, Bang YJ. (2004). DNA hypermethylation in gastric cancer. Aliment Pharmacol Ther., 20 (Suppl 1):131-42.

34. Park JH, Jung Y, Kim TY, Kim SG, Jong HS, Lee JW, Kim DK., Lee JS, Kim NK, Kim TY., Bang Y.J. (2004). Class I Histone Deacetylase-Selective Novel Synthetic Inhibitors Potently Inhibit Human Tumor Proliferation. Clin Cancer Res., 10(15):5271-81.

35. Choi MC, Jong HS, Kim TY, Song SH, Lee DS, Lee JW, Kim TY, Kim NK, Bang YJ. (2004). AKAP12/Gravin is inactivated by epigenetic mechanism in human gastric carcinoma and shows growth suppressor activity. Oncogene, 23(42):7095-103.

36. Park J, Song SH, Kim TY, Choi MC, Jong HS, Kim TY, Lee JW, Kim NK, Kim WH, Bang YJ. (2004), Aberrant methylation of integrin beta 4 gene in human gastric cancer cells. Oncogene, 23(19):3474-80.

37. Kim TY, Lee HS, Hwang KS, Lee, M, Kim JW, Bang YJ, and Kang GH. (2004). Methylation of Runx3 in various types of human cancers and premalignant stages of gastric carcinoma. Lab Invest. , 84(4):479-84.

38. Kim TY, Jong HS, Song SH, Dimtchev A, Jeong SJ, Lee JW, Kim TY, Kim NK, Jung, M, and Bang Y.J. (2003). Transcriptional silencing of the DLC-1 tumor suppressor gene by epigenetic mechanism in gastric cancer cells Oncogene, 22(25), 3943-3951.

39. Park JH, Kim TY, Jong HS, Kim TY, Chun YS, Park JW, Lee CT, Jung HC, Kim NK, and Bang YJ. (2003). Gastric Epithelial Reactive Oxygen Species Prevent Normoxic Degradation of Hypoxia-Inducible factor-1 alpha in Gastric Cancer Cells. Clin. Cancer Res., 9(1), 433-440. 

Curriculum Vitae (last updated: 02-25-2022)

PERSONAL AND PROFESSIONAL INFORMATION                           

Name: Ki Jung Kim, Ph.D.

Institute for Basic Science: Senior Researcher

Professional Address: Center for Cognition and Sociality

                                     Institute for Basic Science

                                     55, Expo-ro, Yuseong-gu, Daejeon, 34126

                                     Republic of Korea

Telephone: +82-10-8371-5855, Fax: +82-42-878-9151

E-mail: kjkim@ibs.re.kr

Google Scholar: Ki Jung Kim

EDUCATION                                                              

Kyung Hee University, Yongin-si, Kyeongggi-do, South Korea

Bachelor of Science in Genetic Engineering (1996-2000)

Catholic University of Korea, College of Medicine, Seoul, South Korea

Master of Science in Neuropharmacology (2002-2004)

Advisor: Prof. Ki-Wug Sung (MD., Ph.D.)

Catholic University of Korea, College of Medicine, Seoul, South Korea

Ph.D in Neurobiology (2004-2009)

Advisor: Prof. Ki-Wug Sung (MD., Ph.D.)

RESEARCH AND PROFESSIONAL EXPERIENCE                             

Research Assistance (Sep. 2002-Apr. 2009)

Department of Pharmacology

Catholic University of Korea, College of Medicine

Seoul, South Korea

Postdoctoral Associate (Jun. 2009-Aug. 2019)

Department of Physiology

Medical College of Georgia

Augusta University, GA, USA

Supervisor: Dr. Jessica A. Filosa

Senior Research Associate (Sep. 2019- Jun. 2021)

Department of Physiology

Medical College of Georgia

Augusta University, GA, USA

Principal Investigator: Dr. Jessica A. Filosa

Senior Researcher (Jul. 2021 - Present)

Center for Cognition and Sociality

Institute for Basic Science

55, Expo-ro, Yuseong-gu, Daejeon, 34126

Republic of Korea

SCIENTIFIC PUBLICATIONS                                               

PEER REVIEW ARTICLES

PUBLISHED

1. Ki Jung Kim, Juan Ramiro Diaz, Jessica L. Presa, Robinson Muller, Michael W. Brands, Mohammad B. Khan, David C. Hess, and Jessica A. Filosa. (2021) Decreased parenchymal arteriole tone in a mouse model of vascular cognitive impairment uncouples vessel-to-neuronal communication. GeroScience. 2021 Jan 7. (https://doi.org/10.1007/s11357-020-00305-x)

2. *Ramiro-Diaz, JM., *Kim, KJ., Brands, M and Filosa, JA. (2019) Augmented astrocyte microdomain Ca²⁺ dynamics and parenchymal arteriole tone in angiotensin II-infused hypertensive mice. Glia. 2018 Dec 2. PMID: 30506941. (*Co-First Author).

1. This article was recommended in F1000 Prime.

1. Ki Jung Kim, Seung Hyun Jeun and Ki-Wug Sung (2017) Lamotrigine, an antiepileptic drug, inhibits 5-HT3 receptor currents in NCB-20 neuroblastoma cells. Korean J Physiol Pharmacol. Mar;21(2):169-177. PMID: 28280410.

2. Kim, KJ., Ramiro-Diaz JM., Iddings, JA., Filosa, JA. (2016) Vasculo-Neuronal Coupling: Retrograde Vascular Communication to Brain Neurons. J Neurosci. 36(50):12624-12639. PMID: 27821575.

1. This article was recommended in Faculty1000.

1. Kim, KJ., Iddings, JA., Stern, JE., Blanco, V.M., Kirov, SA., Croom, D., Filosa, JA. (2015) Astrocyte contribution to flow/pressure-evoked parenchymal arteriole vasoconstriction. J Neurosci. 27;35(21):8245-57. PMID:26019339.

2. Iddings, J.A., Kim, KJ., Zhou, Y., Higashimori, H., Filosa, J.A. (2015) Enhanced parenchymal arteriole tone and astrocyte signaling protect neurovascular coupling mediated parenchymal arteriole vasodilation in the spontaneously hypertensive rat. Journal of Cerebral Blood Flow and Metabolism. 35 (7), 1127-1136. PMID:25757753.

3. Ki Jung Kim, and Jessica A. Filosa. (2012) Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation. Journal of Physiology Apr 1;590(Pt 7):1757-70. PMID 22310311. 

1. A perspective article was writing on this paper by Robert Bryan Jr. JPHYSIOL/2012/233718 "No matter - It matters how you slice it".

1. HyeongSeok Cho, Seung Hyun Jeun, QuingZhong Li, Ki Jung Kim and Ki-Wug Sung. (2012) Involvement of the endocannabinoid system in ethanol-induced corticostriatal synaptic depression. J Pharmacol Sci. 120, 45-49. PMID: 22971846.

2. QuingZhong Li, HyeongSeok Cho, Seung Hyun Jeun, Ki Jung Kim and Ki-Wug Sung. (2011) Effects of Grape Seed Proanthocyanidin on 5-Hydroxytryptamine3 Receptors in NCB-20 Neuroblastoma Cells. Biol Pharm Bull. 34(7), 1109-1115. PMID: 21720021.

3.  Jeun, SH., Cho, HS., Kim, KJ., Li, QZ., Sung, K-W. (2009) Electrophysiological Characterization of AMPA and NMDA Receptors in Rat Dorsal Striatum.Korean J Physiol Pharmacol. Jun;13(3):209-14. PMID: 19885039.

Education

March 2002 - August 2010           B.A. Korea University

ü  Major: Business & Administration

ü  Minor: Law

September 2013 - present               Ph.D. course, KU-KIST Graduate School of Converging Science and Technology

ü  Major: NBIT (Advanced educated in Neuroscience)

Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS)

Research Experiments

October 2012 - August 2013          Internship at KIST

ü  Center for Functional Connectomics

Experiment Techniques

Slice patch-clamp recording (Neuron, Astrocyte), Cell patch (Astrocyte, HEK293T), Sniffer patch, Ca²⁺ imaging, Primary astrocyte culture, Primary neuron culture, Immunohistochemistry, Immunocytochemistry, Confocal microscope imaging, SIM imaging, Imaris 3D rendering, PCR, RT-PCR, DNA/RNA prep, Cloning, Sub-cloning, Stereotaxic surgery (Mouse, Monkey)

Research Interests

ü  Transdiffernentiation, Treating Spinal Cord Injury (SCI) and neurodegenerative diseases with direct reprogramming of reactive astrocyte into neuron

ü  Bestrophin1 channel expression related synaptic property

Publications

1.        Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain, Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215

2.        Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient, Junsung Woo, Young-Eun Han, Wuhyun Koh, Joungha Won, Min Gu Park, Heeyoung An, C. Justin Lee, Exp Neurobiol. 2019 Feb;28(1):30-42

3.        Expression of µ-Opioid Receptor in CA1 Hippocampal Astrocytes, Min-Ho Nam, Kyung-Seok Han, Jaekwang Lee, Jin Young Bae, Heeyoung An, Seahyung Park, Soo-Jin Oh, Eunju Kim, Eunmi Hwang, Yong Chul Bae, C. Justin Lee, Exp Neurobiol. 2018 Apr; 27(2): 120–128.

4.        Control of motor coordination by astrocytic tonic GABA release through modulation of excitation/inhibition balance in cerebellum, Woo J, Min JO, Kang DS, Kim YS, Jung GH, Park HJ, Kim S, An H, Known J, Kim J, Shim I, Kim HG, Lee CJ, Yoon BE, Proc Natl Acad Sci U S A. 2018 Apr 24. pii: 1721187115. do (2018)

5.        Cografting astrocytes improves cell therapeutic outcomes in a Parkinson`s disease model, Song JJ, Oh SM, Kwon OC, Wulansari N, Lee HS, Chang MY, Lee E, Sun W, Lee SE, Chang S, An H, Lee CJ, Lee SH, J Clin Invest 2018 Jan 2;128(1):463-482. (2018)

6.        Astrocytic water channel aquaporin-4 modulates brain plasticity in both mice and humans: a potential gliogenetic mechanism underly, J Woo, JE Kim, JJ Im, J Lee, HS Jeong, S Park, S-Y Jung, H An, S Yoon, SM Lim, S Lee, J Ma, EY Shin, Y-E Han, B Kim, EH Lee, L Feng, H Chun, B-E Yoon, I Kang, SR Dager, IK Lyoo and CJ Lee, Molecular Psychiatry advance online publication 27 June 2017; doi:10.1038/mp.2017.113 (2017)

7.        Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocytes, Jea Kwon, Heeyoung An, Moonsun Sa, Joungha Won,Jeong Im Shin and C. Justin Lee, Exp Neurobiol 2017 Feb;26(1):42-54. (2017)

8.        Differential Cellular Tropism of Lentivirus and Adeno-Associated Virus in the Brain of Cynomolgus Monkey, An H, Cho DW, Lee SE, Yang YS, Han SC, Lee CJ, Exp Neurobiol 2016 Feb;25(1):48-54. doi: 10.5607/en.2016.25.1.48. (2016)

9.        Networked neural spheroid by neuro-bundle mimicking nervous system created by topology effect, Jeong GS, Chang JY, Park JS, Lee SA, Park D, Woo J, An H, Lee CJ, Lee SH, Mol Brain 2015 Mar 22;8(1):17. doi: 10.1186/s13041-015-0109-y. (2015)

10.    Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors, Park H, Han KS, Seo J, Lee J, Dravid SM, Woo J, Chun H, Cho S, Bae J, An H, Koh W, Yoon BE, Berlinguer-Palmini R, Mannaioni G, Traynelis SF, Bae Y, Choi SY, Lee CJ, Mol Brain 2015 Feb 3;8(1):7. (2015)

11.    Glial GABA, synthesized by monoamine oxidase B, mediates tonic inhibition. Bo-Eun Yoon, JunsungWoo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, JooOkMin, Soo-Jin Oh, Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Yong Chul Bae and C. Justin Lee, J Physiol. 2014 Sep 19. DOI: 10.1113 (2014)

Awards

1.        Oral presentation:

                                                                            i.              Direct cell reprogramming of reactive astrocytes into neurons in non-human primate, 2014 KU-KIST symposium

2.        Poster Presentations:

                                                                                          i.             Transdifferentiation of scar-forming reactive astrocytes into functional neurons causes motor recovery after spinal cord injury, 2019 Korea Society for Gene and Cell Therapy

                                                                                        ii.             Cell direct reprogramming of reactive astrocytes into neurons in non-human primates. 2015 Glia Meeting of the Korean Society for Glia Research

Research interest

Neurodegenerative brain disease and scar forming astrocyte

Education

2004-2011 B.A. in Speech Language Pathology, Korean Nazarene University, Republic of Korea

2012-2015 M.S. in Audiology, Catholic University of Daegu, Republic of Korea (advisor: Chul-Hee Choi)

2016-2019 Ph.D. in Medicine (Otology), Dankook University, Republic of Korea (advisor: Jae Yun Jung)

Awards and Honors

2020 Best poster presentation, the 94th annual congress of Korean Society of Otorhinolaryngology-Head and Neck Surgery, Republic of Korea

2019 Travel Award, the 42^th Annual Midwinter Research Meeting of the Association for Research in Otolaryngology (ARO), United States

2018 Best presentation award, 38th Aju otology symposium, Republic of Korea

2017 Best presentation awards, 1st Otology basic research seminar, Korean Otological Society, Republic of Korea

2018 Best presentation award, Korean Society for Laser Medicine and Surgery, Republic of Korea

Grants

2020.09 – 2023.05

Title: Investigation on the role of Ca buffer protein in noise-induced cochlear synaptopathy, National Research Foundation of Korea (NRF), 180,000k KRW

2019.09 – 2020.08

Title: Neuroprotective effect of photobiomodulation mechanism study in secondary ganglion neuron degeneration model, National Research Foundation of Korea (NRF), 45,000k KRW

Publications

1. Lee JH, Carpena NT, Kim s, Lee MY, Jung JY, Choi JE. Photobiomodulation at a wavelength of 633 nm leads to faster functional recovery than 804 nm after facial nerve injury. Journal of Biophotonics. 2021 Jul 12:e202100159. (Front cover image)

2. Kim JH, Jeon S, Choi HD, Lee JH, Bae JS, Kim N, Kim HG, Kim KB, Kim HR. Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence. Journal of Toxicology and Environmental Health, Part A. 2021 Jul 3:1-2.

3. Kim MT, Lee JH, Carpena NT, Lee MY, Chung PS, Jung JY. Estrogen replacement reduces hearing threshold shifts and cochlear hair cell loss after acoustic overexposure in ovariectomized rats. Clinical and Experimental Otorhinolaryngology. 2021 Feb;14(1):61. (co-first author)

4. Lee JH, Lee MY, Choi JE, Jung JY. Auditory Brainstem Response to Paired Click Stimulation as an Indicator of Peripheral Synaptic Health in Noise-Induced Cochlear Synaptopathy. Frontiers in Neuroscience. 2021;14: 596670.

5. Lee JH, Jung JY, Park I. A Gap Prepulse with a Principal Stimulus Yields a Combined Auditory Late Response. Journal of Audiology & Otology. 2020 Jul;24(3):149.

6. Lee JH, Lee MY, Chung PS, Jung JY. Photobiomodulation using low‐level 808 nm diode laser rescues cochlear synaptopathy after acoustic overexposure in rat. Journal of Biophotonics. 2019 Nov;12(11):e201900145.

7. Choi JE, Lee JH, Chang SY, Lee MY, Jung JY. Clinical Implications of Poloxamer 407 as Packing Material in an Animal Model. Audiology and Neurotology. 2019;24(2):100-8.

8, Lee JH, Kim S, Jung JY, Lee MY. Applications of photobiomodulation in hearing research: from bench to clinic. Biomedical Engineering Letters. 2019 Aug;9(3):351-8.

9. Kim JH, Huh YH, Lee JH, Jung JY, Ahn SC, Kim HR. Early exposure to radiofrequency electromagnetic fields at 1850 MHz affects auditory circuits in early postnatal mice. Scientific Reports. 2019 Jan 23;9(1):1-0.

10. Lee JH, Lee MY, Chung PS, Jung JY. Secondary degeneration of auditory neurons after topical aminoglycoside administration in a Gerbil model. Biomed Research International. 2018 Mar 1;2018.

11. Lee MY, Bae SH, Chang SY, Lee JH, Kim SH, Ahn JC, Chung PS, Moy W, Jung JY. Photobiomodulation by laser therapy rescued auditory neuropathy induced by ouabain. Neuroscience Letters. 2016 Oct 28;633:165-73.

12. Lee JH, Chang SY, Moy WJ, Oh C, Kim SH, Rhee CK, Ahn JC, Chung PS, Jung JY, Lee MY. Simultaneous bilateral laser therapy accelerates recovery after noise-induced hearing loss in a rat model. PeerJ. 2016 Jul 21;4:e2252.

Contact: jeakwon@ibs.re.kr (official) onlytojay@gmail.com (private)
Pubmed: https://www.ncbi.nlm.nih.gov/pubmed/?term=jea+kwon
Github: https://github.com/jeakwon/

EDUCATION
[2007.03 ~ 2012.03] Full Scholarship Korea - Japan Joint Government Program
[2008.04 ~ 2012.03] B.E. in Electrical and Electronic Systems at Saitama University, Japan
[2013.08 ~ present] Ph.D. course, KU-KIST Graduate School of Converging Science and Technology in Korea University
[2014.03 ~ 2018.11] Researcher, Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Republic of Korea
[2017.03 ~ present] Technical Research Personnel (Mandatory military service in Republic of Korea)
[2018.12 ~ present] Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS), Republic of Korea

INTERESTS
- Astrocyte-Neuron Interaction
- Circadian Rhythm and Sleep
- Synaptic Plasticity
- Cognition, Human intelligence
- Brain-inspired Artificial Intelligence
 
Skills
- Live cell/slice electrophysiology
- Live cell/slice imaging
- EEG/Circadian rhythm recording
- Python/Matlab programing for data analysis (imaging, electrophysiology, behavior)
- Python programing for machine learning


PUBLICATIONS
Ultrasonic Neuromodulation via Astrocytic TRPA1. Oh SJ, Lee JM, Kim HB, Lee J, Han S, Bae JY, Hong GS, Koh W, Kwon J, Hwang ES, Woo DH, Youn I, Cho IJ, Bae YC, Lee S, Shim JW, Park JH, Lee CJ. Curr Biol. 2019 Oct 21;29(20):3386-3401.e8. doi: 10.1016/j.cub.2019.08.021. Epub 2019 Oct 3.

Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ. Exp Neurobiol. 2019 Apr;28(2):183-215. doi: 10.5607/en.2019.28.2.183. Epub 2019 Apr 30.

Control of motor coordination by astrocytic tonic GABA release through modulation of excitation/inhibition balance in cerebellum. Woo J, Min JO, Kang DS, Kim YS, Jung GH, Park HJ, Kim S, An H, Kwon J, Kim J, Shim I, Kim HG, Lee CJ, Yoon BE. Proc Natl Acad Sci U S A. 2018 May 8;115(19):5004-5009. doi: 10.1073/pnas.1721187115. Epub 2018 Apr 24.

Development of a Low-cost, Comprehensive Recording System for Circadian Rhythm Behavior. Kwon J, Park MG, Lee SE, Lee CJ. Exp Neurobiol. 2018 Feb;27(1):65-75. doi: 10.5607/en.2018.27.1.65. Epub 2018 Feb 28.

Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocytes. Kwon J, An H, Sa M, Won J, Shin JI, Lee CJ. Exp Neurobiol. 2017 Feb;26(1):42-54. doi: 10.5607/en.2017.26.1.42. Epub 2017 Feb 9.

The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons. Ha GE, Lee J, Kwak H, Song K, Kwon J, Jung SY, Hong J, Chang GE, Hwang EM, Shin HS, Lee CJ, Cheong E. Nat Commun. 2016 Dec 19;7:13791. doi: 10.1038/ncomms13791.

EDUCATION

MAR 2014~ present     Ph.D. course, KU-KIST Graduate School of Converging Science and Technology, Seoul, Korea (Thesis advisor: Dr. Changjoon Justin Lee)

MAR 2006~ FEB 2011   B.A. College of Life science & Biotechnology, Korea University, Seoul, Korea

RESEARCH EXPERIENCE

NOV 2018~ Present     Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Korea

FEB 2014~ NOV 2018    Researcher, Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul, Korea

RESEARCH INTERSTS

Neuron-glia interaction

Reactive astrocytes in hypothalamus, Obesity and metabolism

Learning and memory, ADHD

EXPERIMENTAL TECHNIQUES

Slice patch recording (Neuron), Cell patch (COS-7, HEK293T), Ca2+ Imaging

Confocal microscope imaging, Immunohistochemistry, Immunocytochemistry

Primary astrocyte culture, PCR, RT-PCR, DNA/RNA prep., Cloning

Stereotaxic surgery, Open field test, Elevate plus maze, Passive avoidance test, Eight arm radial maze

AWARDS

AUG 2018        BEST PRESENTATION AWARD at 21st KSBNS Annual Meeting

                    (Poster title: GABA from Reactive Astrocytes in Hypothalamus via Monoamine Oxidase B Causes and Exacerbates Obesity)

NOV 2016        Second Award at KIST-BSI Conference 2016

                    (Poster title: GABA from Reactive Astrocytes may Cause and Exacerbate Obesity)

SEP 2016        Second Award at 2016 Converging Conference for KIST-School Ties

                    (Poster title: GABA from Reactive Astrocytes may Cause and Exacerbate Obesity)

PUBLICATION

1.   NaHye Lee*, Moonsun Sa*, Yu Ri Hong, C. Justin Lee†, and JaeHyung Koo†. Fatty Acid Increases cAMP-dependent Lactate and MAO-B-dependent GABA Production in Mouse Astrocytes by Activating a Gαs Protein-coupled Receptor. Experimental Neurobiology, 2018 Oct;27(5):365-376. doi: 10.5607/en.2018.27.5.365.

2.   Jea Kwon, Heeyoung An, Moonsun Sa, Joungha Won, Jeong Im Shin, and C. Justin Lee. Orai1 and Orai3 in combination with stim1 mediate the  majority of store-operated calcium entry in astrocytes. Experimental Neurobiology, 2017 Feb;26(1):42-54. doi: 10.5607/en.2017.26.1.42.

EDUCATION

- 2007.03 ~ 2014.02 : B.A. in Yonsei University

- 2016.03 ~ Present : Ph.D Course (KU-KIST, Advisor: C. Justin Lee)

RESEARCH EXPERIENCES

- 2012.07 ~ 2013.06 Undergraduate Intern (Prof. Park in Yonsei University, Immunology)

- 2014.03 ~ 2014.08 Undergraduate Intern (Prof. Lee in Yonsei University, Bioinformatics)

- 2014.10 ~ 2016.02 Internship (Dr. C. Justin Lee's lab, Glioscience)

PUBLICATIONS

- Yong Kyoung Yoo, Jaekwang Lee, Jinsik Kim, Gangeun Kim, Sunpil Kim, Jeongyeon Kim, Heejung Chun, Jeong Hoon Lee, C. Justin Lee & Kyo Seon Hwang, Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor, Scientific Reports, 2016.

EXPERIMENTAL TECHNIQUES

- Imaging:

  Acute brain slice imaging (Calcium, Fura-2 and various sensors)

  2-photon brain slice imaging

- Behavior:

  Social behaviors (3-chamber test and five-trial social memory test)

  General behaviors (Open field test, novel object recognition and novel place recognition test)

  Fear-associated behavior (Passive avoidance test and contextual fear conditioning)

- Electrophysiology:

  Patch clamping (Cultured cell and acute brain slice)

- Molecular biology:

  Cloning, RT-PCR and Western blot

- Primary astrocyte culture and microporation

- Stereotaxic surgery :

  Guide cannulation (Microdialysis)

  Virus injection

- Immunohistochemistry (IHC) for mouse

- Microdialysis

AWARDS

- Outstanding presentation award (2018 5th SGFP Annual Conference)

EDUCATION

• 2010.03 ~ 2014.08 B.A. in Sookmyung Women’s University

• 2015.03 ~ KU-KIST Graduate School of Converging Science and Technology, Korea University

RESEARCH EXPERIENCES

• 2012.01 ~ 2012.12 Undergraduate intern (Prof. Jonghoon Park, Sookmyung Women’s University)

• 2013.06 ~ 2013.09 52^nd National Undergraduate Symposium on Biology (Aging & Senescence)

EXPERIMENTAL TECHNIQUES

• Electrophysiology: Cell patch, Slice patch

• Imaging: Calcium imaging, Intrinsic optical signal (IOS) imaging

• Molecular biology: Cloning, RT-PCR, Western blot, Biotinylation, Co-immunoprecipitation

• Cellular biology: Transfection, Microporation

• Primary culture: Astrocyte cell culture, Cochlea tissue culture

• Stereotaxic surgery (mouse)

• Immunohistochemistry, Immunocytochemistry

PUBLICATIONS

1. Han K, Lee M, Lim HK, Jang MW, Kwon J, Lee CJ, Kim SG, Suh M. Excitation-inhibition imbalance leads to alteration of neuronal coherence and neurovascular coupling under acute stress. J Neurosci. 2020 Oct 20:JN-RM-1553-20. doi: 10.1523/JNEUROSCI.1553-20.2020. PMID: 33087471.

    

2. Woo J, Jang MW, Lee J, Koh W, Mikoshiba K, Lee CJ. The molecular mechanism of synaptic activity-induced astrocytic volume transient. J Physiol. 2020 Jul 24. doi: 10.1113/JP279741. PMID: 32706443.

    

3. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ. Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Exp Neurobiol. 2019 Apr;28(2):183-215. doi: 10.5607/en.2019.28.2.183. Epub 2019 Apr 30. PMID: 31138989.

AWARDS

Oral Presentation:

• 2017 Brain Science Institute Symposium (The novel channel, AUDITORIN discloses a new class of hearing loss, auditory gliopathy)

Poster Presentation:

• 2018 Glia Meeting of the Korean Society for Glia Research (Human speech perception by AUDITORIN (TMEM43) in cochlear glia)

• 2019 7th SIPF-SIF International Workshop on: Structure and Function of Ion Channels and Transporters (AUDITORIN (TMEM43), A Novel Passive Conductance Channel of Cochlear Glia is Critical for Maintenance of Speech Discrimination)

EDUCATION 

- 2009.03 ~ 2014.03: B.S. in Korea University, Seoul, Republic of Korea
- 2015.03 ~ 2022.08: Ph.D course in KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea

ACADEMIC RESEARCH INTEREST

- Neuroscience (Neuron-glia interaction)
- Neurodegenerative diseases
- Systemic inflammation
- Reactive oxygen species (ROS)
-Drug development
- Post-traumatic stress disorder (PTSD)
- Emotions

EXPERIMENTAL TECHNIQUES 

- Electrophysiology: Cell patch, Slice patch 
- Imaging: Calcium imaging, Neurotransmitters, sensor probe
- Immunohistochemistry, Immunocytochemistry
- Molecular biology: Cloning, qRT-PCR, shRNA 
- Cellular biology: Transfection, Microporation 
- Astrocyte cell culture
- Behavior test (mouse) 
- Stereotaxic surgery (mouse, rat): virus injection, cannula surgery 
- Drug screening assay (H₂O₂, MAOB etc.)
- RNA-Sequencing

AWARD and HONORS

■ Oral Presentation award 
 - 2019 Korean Chemical Society, Division of Medicinal Chemistry; Best Presentation

■ Poster Presentation award
 - 2018 Glia Meeting of the Korean Society for Glia Research
 - 2019 Cold Spring Harbor Asia (CSHA) meeting, Francis Crick Symposium-Transforming Neurosciences:
Questions & Experiments
- 2021 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation Award
- 2022 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation Award
- 2022 IBS-UNIST Symposium; Best Presentation Award

PATENTS 

■ Composition for prevention or treatment of rheumatoid arthritis (10-2020-0120924)

■ NOVEL AMINOAROMATIC COMPOUNDS OR PHARMACEUTICALLY ACCEPTABLE SALT THEREOF AND PHARMACEUTICAL COMPOSITION FOR PREVENTION OR TREATMENT OF NEURODEGENERATIVE DISEASES COMPRISING THE SAME AS AN ACTIVE INGREDIENT (10-2021-0155076)

■ Prolonging lifespan with long-term administration of novel H2O2 scavenger (10-2022-0092661)

PUBLICATION

1. Won W, Choi HJ, Yoo JY, Kim D, Kim TY, Ju YH, Park KD, Lee H, Jung SY, Lee CJ. inhibiting peripheral and central MAO-B ameliorates both joint inflammation and cognitive impairment in rheumatoid arthritis. Exp Mol Med (in press)

2. An H, Lee H, Yang S, Won W, Lee CJ, Nam MH. Adenovirus-induced Reactive Astrogliosis Exacerbates the Pathology of Parkinson's Disease. Exp Neurobiol. 2021 Jun 30;30(3):222-231. doi: 10.5607/en21013. PMID: 34045369; PMCID: PMC8278136.

3. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim ST, Lee CJ. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med 53, 956–972 (2021).

4. Chun H, Im H, Kang YJ, Kim Y, Shin JH,Won W, Lim J, Ju Y, Park YM, Kim S, Lee SE, Lee J, Woo J, Hwang Y, Cho H, Jo S, Park JH, Kim D, Kim DY, Seo JS, Gwag BJ, Kim YS, Park KD, Kaang BK, Cho H, Ryu H, Lee CJ. Severe reactive astrocytes precipitate pathological hallmarks of Alzheimer's disease via H2O2- production. Nat Neurosci. 2020 Dec;23(12):1555-1566. doi: 10.1038/s41593-020-00735-y. Epub 2020 Nov 16. PMID: 33199896.

5. Nam MH, Cho J, Kwon DH, Park JY, Woo J, Lee JM, Lee S, Ko HY, Won W, Kim RG, Song H, Oh SJ, Choi JW, Park KD, Park EK, Jung H, Kim HS, Lee MC, Yun M, Lee CJ, Kim HI. Excessive Astrocytic GABA Causes Cortical Hypometabolism and Impedes Functional Recovery after Subcortical Stroke.Cell Rep. 2020 Jul 7;32(1):107861. doi: 10.1016/j.celrep.2020.107861. Erratum in: Cell Rep. 2020 Jul 21;32(3):107975. PMID: 32640227.

6. Nam MH*, Won W*, Han KS, Lee CJ. Signaling mechanisms of μ-opioid receptor (MOR) in the hippocampus: disinhibition versus astrocytic glutamate regulation. Cell Mol Life Sci. 2021 Jan;78(2):415-426. doi: 10.1007/s00018-020-03595-8. Epub 2020 Jul 15. PMID: 32671427.

- Ph.D course (KU-KIST)

-Glia-neuron interaction, Neurodegeneration and Neuroinflammation.

- wwj@ibs.re.kr

Educational background

- 2016/09 ~ Present, Ph.D. candidate in KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea (Advisor: C. Justin, Lee)

- 2010/03 ~ 2016/08, B.E. in Biomedical engineering, Korea University, Seoul, Republic of Korea

- 2012/05 ~ 2014/02, KATUSA soldier program (U.S. Army Garrison Casey, South Korea)

Research Experiences

- 2014/09 ~ 2016/05, Undergraduate internship, Prof. Sang-Hoon Lee’s iBML lab, Korea University.

Field of interest

- Targeting astrocytes as a novel therapeutic target for treating epilepsy.

- Physiological and pathological function of TRIM9 in astrocytes.

- Modulation of synaptic transmission by astrocytes.

- Mechanosensitive calcium channels on radial glial cells.

EXPERIMENTAL TECHNIQUES

- Calcium imaging, GABA imaging

- Behavior test (Passive avoidance test, Open field test, novel object recognition and novel place recognition test)

 - Electrophysiology: Patch clamping (Cultured cell and acute brain slice), Field recording

- Molecular biology: Cloning, RT-PCR and Western blot

- Primary astrocyte culture

- ICC and IHC

- Virus injection

Publications

-Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive calcium channel. Park MG, Jang H, Lee SH, Lee CJ, Exp Neurobiol 2017 Apr;26(2):71-81. (2017)

- Development of a low-cost, comprehensive recording system for circadian rhythm behavior. Jea Kwon, Min Gu Park, Seung Eun Lee, and C. Justin Lee. Exp Neurobiol. 2019 Feb;28(1):30-42 (2018)

- Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient. Woo J, Han YE, Koh W, Won J, Park MG, An H, Lee CJ, Exp Neurobiol 2018 Feb 27;27(1):65-75; (2018)

- Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215 (2019)

Awards

- 2017/03 ~ Present, Global Ph.D. Fellowship program from National Research Foundation of Korea

EDUCATION

-2013.03 ~ 2017.08 B.A in Seoul National University

-2017.09 ~ Present Ph.D candidate in University of Science and Technology(UST)

RESEARCH EXPERIENCE

-2016.06 ~ 2016.08 Students intern researcher program(학생인턴연구원) in School of Medicine, Seoul National University.

-2017.03 ~ 2017.08 Undergraduate intern in Dr. C. Justin Lee lab, Korea Institute of Science and Technology (KIST)

EXPERIMENTAL TECHNIQUES

-Molecular biology: Cloning, RT-PCR

-Cellular biology: Transfection, Microporation

-Astrocyte cell culture (+ Long-term culture)

-Stereotaxic surgery (mouse)

-Immunohistochemistry

-Imaging: Calcium imaging in vitro

-Electrophysiology: Cell patch, Slice patch

-Behavior test: Passive avoidance test, Open field test, Novel object (Place) recognition test, Context fear conditioning test, Elevated plus maze test, Forced swim test

AWARDS

-Poster presentation

2018 Glia Meeting of the Korean Society for Glia Research (Charcterization of hGFAP-CreERT2 [Tg(GFAP-Cre/ERT2)13Kdmc] in various brain region)

PUBLICATION

-Koh, W., Park, Y. M., Lee, S. E., & Lee, C. J. (2017). AAV-mediated astrocyte-specific gene expression under human ALDH1L1 promoter in mouse thalamus. Experimental neurobiology, 26(6): 350-361.

- Park, Y. M., Chun, H., Shin, J. I., & Lee, C. J. (2018). Astrocyte Specificity and Coverage of hGFAP-CreERT2 [Tg (GFAP-Cre/ERT2) 13Kdmc] Mouse Line in Various Brain Regions. Experimental neurobiology, 27(6): 508-525.

Education

PhD course, Department of Basic Science, IBS School in University of Science and Technology (UST), KOREA (2017.03-present)

M.S, Department of Mathematics, Konkuk University, KOREA (2013.09-2015.08)

B.S, Department of Mathematics, Konkuk University, KOREA (2009.03-2013.08)

Research experiences

2015.07~2016.08 rare cancer branch in National Cancer Center (NCC)

2016.10~2018.05 in Korea Institute of Radiological and Medical Sciences (KIRAMS)

Awards

2015 Poster Presentation Award from Korean Society for Industrial and Applied Mathematics.

(Mathematical modeling of Glioblastoma cell type specific about FLIP-induced TRAIL resistance)

2014 Poster Presentation Award from Korean Society of Mathematical Biology.

(Mathematical modeling of the AKT-mTOR-p70S6K pathway in regulation of the control of FLIP and TRAIL-induced apoptosis in cancer)

Scholarships

2018-1^st Assistant scholarship from University of Science and Technology (UST), Korea

2013-2^nd ~2015-1^st Graduate teaching Scholarship from Konkuk University, KOREA.

MRIDULA NEERAJ KUMAR BHALLA – CV

Contact details - +82-010-7286-9608, +91 97667-11187 ; bmridula158@ibs.re.kr

Date of Birth                          :               15^th August 1996

Nationality                              :                Indian

Research Interest

Astrocytes and CNS demyelination, Astrocytes in aging

Education

Research Experience

Publication

Research Techniques

Awards, Honors, and Scholarships

Curricular and Extracurricular experience

JinHyeong Joo, Integrated Ph.D course

E-mail : jhjoo@ibs.re.kr (official) / wnwlsgud0430@gmail.com (private)

Tel : +82) 010-6847-3680

RESEARCH INTEREST

• Neurodegenerative Disease & Memory in Astrocyte

   

EDUCATION

• [2021.01 ~ Present] Integrated Ph.D course at Univesity of Science & Technology (UST), Korea,

• [2021.01 ~ Present] Dr. C. Justin Lee’s Lab, Institute of Basic Science (IBS) School of UST,

• [2014.03 ~ 2019.02] B.E. in Ajou University, Applied Chemistry & Biological Engineering

   

RESEARCH EXPERIENCES

• [2020.03 ~ 2020.11] Reseacher in Immunobiome, Inc. in POSTECH biotech center, Pohang

• [2019.08. ~ 2019.08] Internship in POSTECH IRT lab. (Shin Hyeog Im’s lab), Pohang

• [2018.07 ~ 2018.09] Internship in Purification process in CJ 제일제당 laboratory, Suwon

RESEARCH TECHNIQUES

Mouse handling

• Oral gavage, i.p./s.c. injection, Blood collection (Heart, Orbital), Manual heart perfusion, mouse breeding

Behavior Test

• Sociability test, Social novelty test via 3-chamber arena, Marble burying test for evaluate repetitive behavior

Molcular work

• DNA / RNA prep, Gel electrophoresis, PCR, qRT-PCR, genotyping,

Cell work

• FACS

Joon-Ho Kwon

Curriculum Vitae ( Updated: Feb 25, 2022 )

IBS ( Institute for Basic Science )

Center for Cognition and Sociality

55, Expo-ro, Yuseong-gu, Daejeon, 34126 Republic of Korea
Mobile : +82-10-4189-9405

Work Email: kwonjoonho@unist.ac.kr

Feb. 2015              Chonnam National University                                                                                                          Gwangju, Korea

                                 Department of Biological Sciences

                                 B.S. in Biological Sciences

Feb. 2017              Korea University                                                                                                                                        Seoul, Korea 

                                 Division of Life Sciences

                                 Thesis: Physiological interaction between copper and zinc in aspergillus fumigatus.

                                 Advisor: Prof. Cheol-Won Yun

                                 M.S. in Life Sciences

Mar. 2022             UNIST (Ulsan National Institute of Science and Technology)                                                          Ulsan, Korea

~ Present               Department of Biomedical Engineering

Co-Advisor: Dr. C. Justin Lee, Prof. Yoon-Kyoung Cho

Ph.D. course

1. Master’s student, Korea University, Laboratory of  Molecular Microbiology, ( Jan. 2015 ~ Dec. 2016 )

2. Technical Research Personnel, Seoul National University College of Medicine, Laboratory of Molecular Imaging and Therapy ( Jan. 2017 ~ Mar. 2020 )

3. Researcher, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Infection and Immunity Research Laboratory ( Apr. 2020 ~ Jun. 2021 )

4. Researcher, Institute for Basic Science (IBS), Cognitive Glioscience group, Director: Dr. C. Justin Lee’s Lab, Center for Cognition and Sociality ( Jul. 2021 ~ Feb. 2022 )

5. Ph.D. Student,UNIST-IBS Neuroscience scholarship ( Mar. 2022 ~ Present )

1. Kim, M. J., Lee, C. H., Lee, Y., Youn, H., Kang, K. W., Kwon, J. H, ... & Chung, J. K. (2019). Glucose-6-phosphatase Expression–Mediated [18 F] FDG Efflux in Murine Inflammation and Cancer Models. Molecular imaging and biology, 21(5), 917-925.

2. Kang, S., Seo, H., Moon, H. S., Kwon, J. H., Park, Y. S., & Yun, C. W. (2020). The Role of Zinc in Copper Homeostasis of Aspergillus fumigatus. International journal of molecular sciences, 21(20), 7665.

    

1. Joon-Ho Kwon, Cheol-Won Yun, "Physiological interaction between copper and zinc in aspergillus fumigatus", International Meeting of the Federation of Korean Microbiological Societies (2016) – poster

    

    

Month/Year        Title                                                                                          Institution                              

03/2015-09/2015    BK21 PLUS Scholarship (National Funding for Life Sciences)       Korea University

09/2015-09/2016    Teaching Assistant Scholarship                                                         Korea University

General mouse works ( Organ dissection, maintenance, genotyping ), Cell culture (  Fungi, Various cancer cells, primary Thyroid cancer cells ), Cloning, Transfection, Transduction, PCR, Northern blot, RT-qPCR, Western blot, Pull-down assay, Luciferase assay, Cryosection and IHC( Immunohistochemistry ), IF( immunofluorescence ), Confocal Microscopy, Tumor ( Breast, Brain ) modeling, Stereotaxic injection, Small animal imaging test ( PET/CT, IVIS ), FACS, ICP-OES.

Jiwoon Lim

Research Interest

Astrocyte’s role in synapse, memory, and disease

Education

Research Experience

Publication

Poster Exhibition

Lim, J.*, Jee, S., Kwon J., Sa M. & Lee, C. J. (2020). Trifluoperazine (TFP) elevates intracellular Ca2+ level and maintains the open state of the store-operated calcium entry (SOCE) in astrocyte. The Korean Society for Brain and Neural Sciences, Seoul, Korea of Republic

Research Techniques

Awards, Honors, and Scholarships