Tai Young Kim

• Senior Researcher

• Cholesteol metablisim in glioblastoma, Indentification of interacting proteins using BioID, Drug development against COVID-19 coronavirus (SARS-CoV-2)

• taik@ibs.re.kr
  

EDUCATION

2002             Ph.D. in Tumor Biology, Seoul National University, College of Medicine, Korea (advisor: Yung-Jue Bang)

1996             M.S. in Microbiology, Seoul National University, Korea (advisor: Gajin Jeong)

1993             B.S. in Microbiology, Hankuk University of Foreign Studies, Korea.

PROFESSIONAL EXPERIENCES

06/2020 – Current    Senior Researcher, Center for Cognitive and Sociality, Institute for Basic Science, Daejeon, Korea

01/2019 – 05/2020   Research Fellow, Cognitive Glioscience Group, Center for Cognitive and Sociality, Institute for Basic Science, Daejeon,

                            Korea

11/2015 – 12/2018   Research Professor, Kyung Hee University Korean Medicine, Korea.

01/2014 – 10/2015   Principal Research Scientist, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National

                            University, Korea.

08/2013 – 12/2013   Research Associate Professor, School of Nano-Bioscience and Chemical Engineering, UNIST, Republic of Korea

01/2009 - 07/2013   Postdoctoral Research Associate, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC

                            (Advisor: Channing J. Der)

01/2004 - 12/2008   Postdoctoral Research Associate, Department of Pharmacology, University of North Carolina, Chapel Hill, NC

                            (Advisor:  Rudy L. Juliano)

08/2002 - 12/2003   Postdoctoral Research Associate, Cancer Research Institute, Seoul National University, Korea (advisor: Yung-Jue Bang)

07/1998 - 03/1999   M.S. Researcher, Cancer Research Institute, Seoul National University, Korea

AWARDS and FELLOWSHIPS

2016-2019    Research Fellow Grant, Ministry of Education, Korea of Republic

2013–2016    Research Fellow Grant, Ministry of Education, Korea of Republic

2009-2011    Susan G. Komen Research Grant

2003            Young Researcher Awards in Seoul National University Cancer Research Institute

PUBLICATION

1. Jang MW, Oh D, Yi E, Liu X, Ling J, Kim N, Sharma K, Kim TY, Lee S, Kim AR, Kim MY, Kim MA, Lee M, Han JH, Han JJ, Park HR, Kim BJ, Lee SY, Woo DH, Oh J, Oh SJ, Du T, Koo JW, Oh SH, Shin HW, Seong MW, Lee KY, Kim UK, Shin JB, Sang S, Cai X, Mei L, He C, Blanton SH, Chen ZY, Chen H, Liu X, Nourbakhsh A, Huang Z, Kang KW, Park WY, Feng Y, Lee CJ, Choi BY (2021) A nonsense TMEM43 variant leads to disruption of connexin-linked function and autosomal dominant auditory neuropathy spectrum disorder. Proc Natl Acad Sci, USA. Jun 1;118(22)

2. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim ST, Lee CJ (2021) Platycodin D, a natural product from Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med, May;53(5):956-972

3. Lee SJ, Choi YJ, Kim HI, Moon HE, Baek SH, Kim TY, Ko SG (2021) LDLR facilitates platycodin D-induced autophagy inhibition.and cell death in glioblastoma cells. Mol Oncol. May 1 *Co-corresponding Author.

4. Kim HI, Lee SJ, Choi YJ, Kim MJ, Kim TY, Ko SG (2021) Quercetin induces apoptosis in glioblastoma cell lines U87MG and U373MG by suppressing Axl/IL-6/STAT3 signaling pathway. Am J Chin Med, Mar 3;1-18. *Co-corresponding Author

5. Choi YJ, Lee SJ, Kim HI, Lee HJ, Kang SJ, Kim TY, Cheon C, Ko SG. (2020) Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells. Sci Rep, Nov 16;10(1):1983. *Co-corresponding Author

6. Won J, Lee S, Park M, Kim TY, Park MG, Choi BY, Kim D, Chang H, Kim VN, Lee CJ (2020) Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19). Exp Neurobiol, Apr 30; 29(2):107-119.

7. Hong SH, Ku JM, Kim HI, Kim TY, Seo HS, Shin YC, Ko SG (2019) Topical Application of KAJD Attenuates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms Through Regulation of IgE and MAPK Pathways in BALB/C Mice and Several Immune Cell Types. Front Pharmacol. Sep 19; 10:1097.

8. Kim HI, Hong SH, Ku JM, Lim YS, Lee SJ, Song J, Kim TY, Cheon C, Ko SG. (2019) Scutellaria Radix Promotes Apoptosis in Non-Small Cell Lung Cancer Cells via Induction of AMPK-Dependent Autophagy. Am J Chin Med. 47(3):691-705

9. Ku JM, Hong SH, Kim SR, Choi HS, Kim HI, Kim DU, Oh SM, Seo HS, Kim TY, c, Cheon C, Ko SG. (2018) Novel mechanism The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo. BMC Complement Altern Med. Jul 13;18(1):215

10. Kim HI, Hong SH, Ku JM, Kang S, Kim TY, Shin YC, Ko SG. (2017) Tonggyu-tang, a traditional Korean medicine, suppresses pro-inflammatory cytokine production through inhibition of MAPK and NF-κB activation in human mast cells and keratinocytes. BMC Complement Altern Med. Mar 31;17(1):186

11. Kim HJ, Kwon S, Nam SH, Jung JW, Kang M, Ryu J, Kim JE, Cheong JG, Cho CY, Kim S, Song DG, Kim YN, Kim TY, Jung MK, Lee KM, Pack CG, Lee JW. (2017) Dynamic and coordinated single-molecular interactions at TM4SF5-enriched microdomains guide invasive behaviors in 2- and 3-dimensional environments.FASEB J. 2017, 31(4):1461-1481.

12. Choi J, Kang M, Nam SH, Lee GH, Kim HJ, Ryu J, Cheong JG, Jung JW, Kim TY, Lee HY, Lee JW (2015) Bidirectional signaling between TM4SF5 and IGF1R promotes resistance to EGFR kinase inhibitors. Lung Cancer, 90(1):22-31

13. Nam SH, Kim D, Lee MS, Lee D, Kwak TK, Kang MK, Ryu J, Kim HJ, Song HE, Choi J, Lee GH, Kim SY, Park SH, Kim DG, Kwon NH, Kim TY, Thiery JP, Kim S, and Lee JW (2015) Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels. Oncotarget, 6(25): 21655-74

14. Lee D, Na J, Ryu J, Kim HJ, Nam SH, Kang M, Jung JW, Lee MS, Song HE, Choi J, Lee GH, Kim TY, Chung JK, Park KH, Kim SH, Kim H, Seo H, Kim P, Youn H, Lee JW (2015) Interaction of tetraspan(in) TM4SF5 with CD44 promotes self-renewal and circulating capacities of hepatocarcinoma cells. Hepatology, 61(6):1978-97

15. Kim TY, Seisser PM, Rossman KL, Goldfarb D, Mackinnon K, Yan F, Yi X, MacCoss, M, Moon RT, Der CJ, Major MB (2015) Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase. Mol Cell Biol, 35(1):167-81

16. Kang M, Ryu J, Lee D, Lee MS, Kim HJ, Nam SH, Song HE, Choi J, Lee GH, Kim TY, Lee H, Kim SJ, Ye SK, Kim S, Lee JW (2014) Correlations between Transmembrane 4 L6 Family Member 5 (TM4SF5), CD151, and CD63 in Liver Fibrotic Phenotypes and Hepatic Migration and Invasive Capacities. PLoS One. 9(7):e102817

17. Lee MS, Kim S, Kim BG, Won C, Nam SH, Kang S, Kim HJ, Kang M, Ryu J, Song HE, Lee D, Ye SK, Jeon NL, Kim TY, Cho NH, Lee JW (2014) Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation. Biochim Biophys Acta. 1843(9):2037-54

18. Kim TY, Jackson S, Xiong Y, Whitsett TG, Lobello JR, Tran NL, Malek NP, Bang YJ, Der CJ (2013) Loss of DLC1 RhoGAP tumor suppressor expression and function by CUL4A-DDB1-FBXW5-dependent ubiquitination and degradation in non-small cell lung cancer cells. Proc Natl Acad Sci, USA. 110(42):16868-73 

19. Vigil D, Kim TY, Plachco A, Garton AJ, Castaldo L, Pachter JA, Dong H, Chen X, Tokar B, Campbell SL, Der CJ (2012) ROCK1 and ROCK2 are required for Non-Small Cell Lung Cancer Anchorage-Independent Growth and Invasion. Cancer Res. 72(20):5338-47

20. Kim TY, Vigil D, Der CJ, Juliano RL (2009) Role of DLC-1, a Tumor Suppressor Protein with RhoGAP activity, in Regulation of the Cytoskeleton and Cell Motility. Cancer Metastasis Reviews28(1-2):77-83.

21. Kim TY, Kim IS, Jong HS, Lee JW, Kim TY, Jung M, and Bang YJ. (2008) Transcriptional Induction of DLC-1 gene through Sp1 sites by histone deacetylase inhibitors in gastric cancer cells. Exp Mol Med 40 (6):639-46.

22. Kim TY, Healy KD, Der CJ, Sciaky N, Bang YJ, Juliano RL (2008) Effects of Structure of Rho GTPase-activating Protein DLC-1 on Cell Morphology and Migration. J Biol Chem 283(47): 32762-70.

23. SchrampM, Ying O, Kim TY, Martin GS. (2008) ERK5 Promotes Src-induced Podosome Formation by limiting Rho activation. J Cell Biol. 181(7):1195-210.

24. Healy KD, Hodgson L, Kim TY, Shutes A, Maddileti S, Juliano RL, Hahn KM, Harden TK, Bang YJ, Der CJ. (2008) DLC-1 Suppresses Non-Small Cell Lung Cancer Growth and Invasion by RhoGAP-Dependent and Independent Mechanisms. Mol Carcinog. 47(5):326-37.

25. Jung Y, Park J, Kim TY, Park JH, Jong HS, Im SA, Robertson KD, Bang YJ, Kim TY. (2007). Potential advantages of DNA methyltransferase 1 (DNMT1)-targeted inhibition for cancer therapy. J Mol Med. 85(10):1137-48.

26. Kim TY, Lee JW, Kim HP, Jong HS, Kim TY, and Bang YJ. (2007) DLC-1, a GTPase-activating protein for Rho, is associated with cell proliferation, morphology, and migration in human hepatocellular carcinoma. Biochem Biophys Res Commun. 355(1):72-7.

27. Lee KW, Kim JH, Park JH, Kim HP, Song SH, Kim SG, Kim TY, Jong HS, Jung KH, Im SA, Kim TY, Kim NK, Bang YJ. (2006). Antitumor Activity of SK-7041, a Novel Histone Deacetylase Inhibitor, in Human Lung and Breast Cancer Cells. Anticancer Res. 26(5A):3429-38.

28. Lee SY, Kim TY, Lee MS, Kim YB, Chung E, Kim S, Lee JW. (2006). Focal adhesion and actin organization by a cross-talk of TM4SF5 with integrin alpha2 are regulated by serum treatment. Exp Cell Res. 312(16):2983-99.

29. Juliano RL, Dixit VR, Kang H, Kim TY, Miyamoto Y, Xu D. (2005). Epigenetic manipulation of gene expression: a toolkit for cell biologists. J. Cell Biol. 169(6):847-57

30. Kim JH, Lee KW, Jung Y, Kim TY, Ham HS, Jong HS, Jung KH, Im SA, Kim TY, Kim NK, Bang YJ. (2005). Cytotoxic effects of pemetrexed in gastric cancer cells. Cancer Sci. 96(6):365-71.

31. Kim HP, Kim TY, Lee MS, Jong HS, Kim TY, Lee JW, Bang YJ. (2005). TGF-1-mediated activations of c-Src and Rac1 modulate levels of cyclins and p27^Kip1 CDK inhibitor in hepatoma cells replated on fibronectin. Biochim Biophys Acta (BBA)-Mol Cell Res. 1743(1-2):151-61.

32. Yim HW, Jong HS, Kim TY, Choi HH, Kim SG, Song SH, Kim J, Ko SG, Lee JW, Kim TY, Bang YJ. (2005). Cyclooxygenase-2 Inhibits Novel Ginseng Metabolite-Mediated Apoptosis. Cancer Res. 65(5):1952-60.

33. Kim TY, Jong HS, Jung Y, Kim TY, Kang GH, Bang YJ. (2004). DNA hypermethylation in gastric cancer. Aliment Pharmacol Ther., 20 (Suppl 1):131-42.

34. Park JH, Jung Y, Kim TY, Kim SG, Jong HS, Lee JW, Kim DK., Lee JS, Kim NK, Kim TY., Bang Y.J. (2004). Class I Histone Deacetylase-Selective Novel Synthetic Inhibitors Potently Inhibit Human Tumor Proliferation. Clin Cancer Res., 10(15):5271-81.

35. Choi MC, Jong HS, Kim TY, Song SH, Lee DS, Lee JW, Kim TY, Kim NK, Bang YJ. (2004). AKAP12/Gravin is inactivated by epigenetic mechanism in human gastric carcinoma and shows growth suppressor activity. Oncogene, 23(42):7095-103.

36. Park J, Song SH, Kim TY, Choi MC, Jong HS, Kim TY, Lee JW, Kim NK, Kim WH, Bang YJ. (2004), Aberrant methylation of integrin beta 4 gene in human gastric cancer cells. Oncogene, 23(19):3474-80.

37. Kim TY, Lee HS, Hwang KS, Lee, M, Kim JW, Bang YJ, and Kang GH. (2004). Methylation of Runx3 in various types of human cancers and premalignant stages of gastric carcinoma. Lab Invest. , 84(4):479-84.

38. Kim TY, Jong HS, Song SH, Dimtchev A, Jeong SJ, Lee JW, Kim TY, Kim NK, Jung, M, and Bang Y.J. (2003). Transcriptional silencing of the DLC-1 tumor suppressor gene by epigenetic mechanism in gastric cancer cells Oncogene, 22(25), 3943-3951.

39. Park JH, Kim TY, Jong HS, Kim TY, Chun YS, Park JW, Lee CT, Jung HC, Kim NK, and Bang YJ. (2003). Gastric Epithelial Reactive Oxygen Species Prevent Normoxic Degradation of Hypoxia-Inducible factor-1 alpha in Gastric Cancer Cells. Clin. Cancer Res., 9(1), 433-440. 

Curriculum Vitae (last updated: 02-25-2022)

PERSONAL AND PROFESSIONAL INFORMATION                           

Name: Ki Jung Kim, Ph.D.

Institute for Basic Science: Senior Researcher

Professional Address: Center for Cognition and Sociality

                                     Institute for Basic Science

                                     55, Expo-ro, Yuseong-gu, Daejeon, 34126

                                     Republic of Korea

Telephone: +82-10-8371-5855, Fax: +82-42-878-9151

E-mail: kjkim@ibs.re.kr

Google Scholar: Ki Jung Kim

EDUCATION                                                              

Kyung Hee University, Yongin-si, Kyeongggi-do, South Korea

Bachelor of Science in Genetic Engineering (1996-2000)

Catholic University of Korea, College of Medicine, Seoul, South Korea

Master of Science in Neuropharmacology (2002-2004)

Advisor: Prof. Ki-Wug Sung (MD., Ph.D.)

Catholic University of Korea, College of Medicine, Seoul, South Korea

Ph.D in Neurobiology (2004-2009)

Advisor: Prof. Ki-Wug Sung (MD., Ph.D.)

RESEARCH AND PROFESSIONAL EXPERIENCE                             

Research Assistance (Sep. 2002-Apr. 2009)

Department of Pharmacology

Catholic University of Korea, College of Medicine

Seoul, South Korea

Postdoctoral Associate (Jun. 2009-Aug. 2019)

Department of Physiology

Medical College of Georgia

Augusta University, GA, USA

Supervisor: Dr. Jessica A. Filosa

Senior Research Associate (Sep. 2019- Jun. 2021)

Department of Physiology

Medical College of Georgia

Augusta University, GA, USA

Principal Investigator: Dr. Jessica A. Filosa

Senior Researcher (Jul. 2021 - Present)

Center for Cognition and Sociality

Institute for Basic Science

55, Expo-ro, Yuseong-gu, Daejeon, 34126

Republic of Korea

SCIENTIFIC PUBLICATIONS                                               

PEER REVIEW ARTICLES

PUBLISHED

1. Ki Jung Kim, Juan Ramiro Diaz, Jessica L. Presa, Robinson Muller, Michael W. Brands, Mohammad B. Khan, David C. Hess, and Jessica A. Filosa. (2021) Decreased parenchymal arteriole tone in a mouse model of vascular cognitive impairment uncouples vessel-to-neuronal communication. GeroScience. 2021 Jan 7. (https://doi.org/10.1007/s11357-020-00305-x)

2. *Ramiro-Diaz, JM., *Kim, KJ., Brands, M and Filosa, JA. (2019) Augmented astrocyte microdomain Ca²⁺ dynamics and parenchymal arteriole tone in angiotensin II-infused hypertensive mice. Glia. 2018 Dec 2. PMID: 30506941. (*Co-First Author).

1. This article was recommended in F1000 Prime.

1. Ki Jung Kim, Seung Hyun Jeun and Ki-Wug Sung (2017) Lamotrigine, an antiepileptic drug, inhibits 5-HT3 receptor currents in NCB-20 neuroblastoma cells. Korean J Physiol Pharmacol. Mar;21(2):169-177. PMID: 28280410.

2. Kim, KJ., Ramiro-Diaz JM., Iddings, JA., Filosa, JA. (2016) Vasculo-Neuronal Coupling: Retrograde Vascular Communication to Brain Neurons. J Neurosci. 36(50):12624-12639. PMID: 27821575.

1. This article was recommended in Faculty1000.

1. Kim, KJ., Iddings, JA., Stern, JE., Blanco, V.M., Kirov, SA., Croom, D., Filosa, JA. (2015) Astrocyte contribution to flow/pressure-evoked parenchymal arteriole vasoconstriction. J Neurosci. 27;35(21):8245-57. PMID:26019339.

2. Iddings, J.A., Kim, KJ., Zhou, Y., Higashimori, H., Filosa, J.A. (2015) Enhanced parenchymal arteriole tone and astrocyte signaling protect neurovascular coupling mediated parenchymal arteriole vasodilation in the spontaneously hypertensive rat. Journal of Cerebral Blood Flow and Metabolism. 35 (7), 1127-1136. PMID:25757753.

3. Ki Jung Kim, and Jessica A. Filosa. (2012) Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation. Journal of Physiology Apr 1;590(Pt 7):1757-70. PMID 22310311. 

1. A perspective article was writing on this paper by Robert Bryan Jr. JPHYSIOL/2012/233718 "No matter - It matters how you slice it".

1. HyeongSeok Cho, Seung Hyun Jeun, QuingZhong Li, Ki Jung Kim and Ki-Wug Sung. (2012) Involvement of the endocannabinoid system in ethanol-induced corticostriatal synaptic depression. J Pharmacol Sci. 120, 45-49. PMID: 22971846.

2. QuingZhong Li, HyeongSeok Cho, Seung Hyun Jeun, Ki Jung Kim and Ki-Wug Sung. (2011) Effects of Grape Seed Proanthocyanidin on 5-Hydroxytryptamine3 Receptors in NCB-20 Neuroblastoma Cells. Biol Pharm Bull. 34(7), 1109-1115. PMID: 21720021.

3.  Jeun, SH., Cho, HS., Kim, KJ., Li, QZ., Sung, K-W. (2009) Electrophysiological Characterization of AMPA and NMDA Receptors in Rat Dorsal Striatum.Korean J Physiol Pharmacol. Jun;13(3):209-14. PMID: 19885039.

Education

March 2002 - August 2010           B.A. Korea University

ü  Major: Business & Administration

ü  Minor: Law

September 2013 - present               Ph.D. course, KU-KIST Graduate School of Converging Science and Technology

ü  Major: NBIT (Advanced educated in Neuroscience)

Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS)

Research Experiments

October 2012 - August 2013          Internship at KIST

ü  Center for Functional Connectomics

Experiment Techniques

Slice patch-clamp recording (Neuron, Astrocyte), Cell patch (Astrocyte, HEK293T), Sniffer patch, Ca²⁺ imaging, Primary astrocyte culture, Primary neuron culture, Immunohistochemistry, Immunocytochemistry, Confocal microscope imaging, SIM imaging, Imaris 3D rendering, PCR, RT-PCR, DNA/RNA prep, Cloning, Sub-cloning, Stereotaxic surgery (Mouse, Monkey)

Research Interests

ü  Transdiffernentiation, Treating Spinal Cord Injury (SCI) and neurodegenerative diseases with direct reprogramming of reactive astrocyte into neuron

ü  Bestrophin1 channel expression related synaptic property

Publications

1.        Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain, Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215

2.        Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient, Junsung Woo, Young-Eun Han, Wuhyun Koh, Joungha Won, Min Gu Park, Heeyoung An, C. Justin Lee, Exp Neurobiol. 2019 Feb;28(1):30-42

3.        Expression of µ-Opioid Receptor in CA1 Hippocampal Astrocytes, Min-Ho Nam, Kyung-Seok Han, Jaekwang Lee, Jin Young Bae, Heeyoung An, Seahyung Park, Soo-Jin Oh, Eunju Kim, Eunmi Hwang, Yong Chul Bae, C. Justin Lee, Exp Neurobiol. 2018 Apr; 27(2): 120–128.

4.        Control of motor coordination by astrocytic tonic GABA release through modulation of excitation/inhibition balance in cerebellum, Woo J, Min JO, Kang DS, Kim YS, Jung GH, Park HJ, Kim S, An H, Known J, Kim J, Shim I, Kim HG, Lee CJ, Yoon BE, Proc Natl Acad Sci U S A. 2018 Apr 24. pii: 1721187115. do (2018)

5.        Cografting astrocytes improves cell therapeutic outcomes in a Parkinson`s disease model, Song JJ, Oh SM, Kwon OC, Wulansari N, Lee HS, Chang MY, Lee E, Sun W, Lee SE, Chang S, An H, Lee CJ, Lee SH, J Clin Invest 2018 Jan 2;128(1):463-482. (2018)

6.        Astrocytic water channel aquaporin-4 modulates brain plasticity in both mice and humans: a potential gliogenetic mechanism underly, J Woo, JE Kim, JJ Im, J Lee, HS Jeong, S Park, S-Y Jung, H An, S Yoon, SM Lim, S Lee, J Ma, EY Shin, Y-E Han, B Kim, EH Lee, L Feng, H Chun, B-E Yoon, I Kang, SR Dager, IK Lyoo and CJ Lee, Molecular Psychiatry advance online publication 27 June 2017; doi:10.1038/mp.2017.113 (2017)

7.        Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocytes, Jea Kwon, Heeyoung An, Moonsun Sa, Joungha Won,Jeong Im Shin and C. Justin Lee, Exp Neurobiol 2017 Feb;26(1):42-54. (2017)

8.        Differential Cellular Tropism of Lentivirus and Adeno-Associated Virus in the Brain of Cynomolgus Monkey, An H, Cho DW, Lee SE, Yang YS, Han SC, Lee CJ, Exp Neurobiol 2016 Feb;25(1):48-54. doi: 10.5607/en.2016.25.1.48. (2016)

9.        Networked neural spheroid by neuro-bundle mimicking nervous system created by topology effect, Jeong GS, Chang JY, Park JS, Lee SA, Park D, Woo J, An H, Lee CJ, Lee SH, Mol Brain 2015 Mar 22;8(1):17. doi: 10.1186/s13041-015-0109-y. (2015)

10.    Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors, Park H, Han KS, Seo J, Lee J, Dravid SM, Woo J, Chun H, Cho S, Bae J, An H, Koh W, Yoon BE, Berlinguer-Palmini R, Mannaioni G, Traynelis SF, Bae Y, Choi SY, Lee CJ, Mol Brain 2015 Feb 3;8(1):7. (2015)

11.    Glial GABA, synthesized by monoamine oxidase B, mediates tonic inhibition. Bo-Eun Yoon, JunsungWoo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, JooOkMin, Soo-Jin Oh, Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Yong Chul Bae and C. Justin Lee, J Physiol. 2014 Sep 19. DOI: 10.1113 (2014)

Awards

1.        Oral presentation:

                                                                            i.              Direct cell reprogramming of reactive astrocytes into neurons in non-human primate, 2014 KU-KIST symposium

2.        Poster Presentations:

                                                                                          i.             Transdifferentiation of scar-forming reactive astrocytes into functional neurons causes motor recovery after spinal cord injury, 2019 Korea Society for Gene and Cell Therapy

                                                                                        ii.             Cell direct reprogramming of reactive astrocytes into neurons in non-human primates. 2015 Glia Meeting of the Korean Society for Glia Research

Research interest

Neurodegenerative brain disease and scar forming astrocyte

Education

2004-2011 B.A. in Speech Language Pathology, Korean Nazarene University, Republic of Korea

2012-2015 M.S. in Audiology, Catholic University of Daegu, Republic of Korea (advisor: Chul-Hee Choi)

2016-2019 Ph.D. in Medicine (Otology), Dankook University, Republic of Korea (advisor: Jae Yun Jung)

Awards and Honors

2020 Best poster presentation, the 94th annual congress of Korean Society of Otorhinolaryngology-Head and Neck Surgery, Republic of Korea

2019 Travel Award, the 42^th Annual Midwinter Research Meeting of the Association for Research in Otolaryngology (ARO), United States

2018 Best presentation award, 38th Aju otology symposium, Republic of Korea

2017 Best presentation awards, 1st Otology basic research seminar, Korean Otological Society, Republic of Korea

2018 Best presentation award, Korean Society for Laser Medicine and Surgery, Republic of Korea

Grants

2020.09 – 2023.05

Title: Investigation on the role of Ca buffer protein in noise-induced cochlear synaptopathy, National Research Foundation of Korea (NRF), 180,000k KRW

2019.09 – 2020.08

Title: Neuroprotective effect of photobiomodulation mechanism study in secondary ganglion neuron degeneration model, National Research Foundation of Korea (NRF), 45,000k KRW

Publications

1. Lee JH, Carpena NT, Kim s, Lee MY, Jung JY, Choi JE. Photobiomodulation at a wavelength of 633 nm leads to faster functional recovery than 804 nm after facial nerve injury. Journal of Biophotonics. 2021 Jul 12:e202100159. (Front cover image)

2. Kim JH, Jeon S, Choi HD, Lee JH, Bae JS, Kim N, Kim HG, Kim KB, Kim HR. Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence. Journal of Toxicology and Environmental Health, Part A. 2021 Jul 3:1-2.

3. Kim MT, Lee JH, Carpena NT, Lee MY, Chung PS, Jung JY. Estrogen replacement reduces hearing threshold shifts and cochlear hair cell loss after acoustic overexposure in ovariectomized rats. Clinical and Experimental Otorhinolaryngology. 2021 Feb;14(1):61. (co-first author)

4. Lee JH, Lee MY, Choi JE, Jung JY. Auditory Brainstem Response to Paired Click Stimulation as an Indicator of Peripheral Synaptic Health in Noise-Induced Cochlear Synaptopathy. Frontiers in Neuroscience. 2021;14: 596670.

5. Lee JH, Jung JY, Park I. A Gap Prepulse with a Principal Stimulus Yields a Combined Auditory Late Response. Journal of Audiology & Otology. 2020 Jul;24(3):149.

6. Lee JH, Lee MY, Chung PS, Jung JY. Photobiomodulation using low‐level 808 nm diode laser rescues cochlear synaptopathy after acoustic overexposure in rat. Journal of Biophotonics. 2019 Nov;12(11):e201900145.

7. Choi JE, Lee JH, Chang SY, Lee MY, Jung JY. Clinical Implications of Poloxamer 407 as Packing Material in an Animal Model. Audiology and Neurotology. 2019;24(2):100-8.

8, Lee JH, Kim S, Jung JY, Lee MY. Applications of photobiomodulation in hearing research: from bench to clinic. Biomedical Engineering Letters. 2019 Aug;9(3):351-8.

9. Kim JH, Huh YH, Lee JH, Jung JY, Ahn SC, Kim HR. Early exposure to radiofrequency electromagnetic fields at 1850 MHz affects auditory circuits in early postnatal mice. Scientific Reports. 2019 Jan 23;9(1):1-0.

10. Lee JH, Lee MY, Chung PS, Jung JY. Secondary degeneration of auditory neurons after topical aminoglycoside administration in a Gerbil model. Biomed Research International. 2018 Mar 1;2018.

11. Lee MY, Bae SH, Chang SY, Lee JH, Kim SH, Ahn JC, Chung PS, Moy W, Jung JY. Photobiomodulation by laser therapy rescued auditory neuropathy induced by ouabain. Neuroscience Letters. 2016 Oct 28;633:165-73.

12. Lee JH, Chang SY, Moy WJ, Oh C, Kim SH, Rhee CK, Ahn JC, Chung PS, Jung JY, Lee MY. Simultaneous bilateral laser therapy accelerates recovery after noise-induced hearing loss in a rat model. PeerJ. 2016 Jul 21;4:e2252.

Contact: jeakwon@ibs.re.kr (official) onlytojay@gmail.com (private)
Pubmed: https://www.ncbi.nlm.nih.gov/pubmed/?term=jea+kwon
Github: https://github.com/jeakwon/

EDUCATION
[2007.03 ~ 2012.03] Full Scholarship Korea - Japan Joint Government Program
[2008.04 ~ 2012.03] B.E. in Electrical and Electronic Systems at Saitama University, Japan
[2013.08 ~ present] Ph.D. course, KU-KIST Graduate School of Converging Science and Technology in Korea University
[2014.03 ~ 2018.11] Researcher, Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Republic of Korea
[2017.03 ~ present] Technical Research Personnel (Mandatory military service in Republic of Korea)
[2018.12 ~ present] Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS), Republic of Korea

INTERESTS
- Astrocyte-Neuron Interaction
- Circadian Rhythm and Sleep
- Synaptic Plasticity
- Cognition, Human intelligence
- Brain-inspired Artificial Intelligence
 
Skills
- Live cell/slice electrophysiology
- Live cell/slice imaging
- EEG/Circadian rhythm recording
- Python/Matlab programing for data analysis (imaging, electrophysiology, behavior)
- Python programing for machine learning


PUBLICATIONS
Ultrasonic Neuromodulation via Astrocytic TRPA1. Oh SJ, Lee JM, Kim HB, Lee J, Han S, Bae JY, Hong GS, Koh W, Kwon J, Hwang ES, Woo DH, Youn I, Cho IJ, Bae YC, Lee S, Shim JW, Park JH, Lee CJ. Curr Biol. 2019 Oct 21;29(20):3386-3401.e8. doi: 10.1016/j.cub.2019.08.021. Epub 2019 Oct 3.

Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ. Exp Neurobiol. 2019 Apr;28(2):183-215. doi: 10.5607/en.2019.28.2.183. Epub 2019 Apr 30.

Control of motor coordination by astrocytic tonic GABA release through modulation of excitation/inhibition balance in cerebellum. Woo J, Min JO, Kang DS, Kim YS, Jung GH, Park HJ, Kim S, An H, Kwon J, Kim J, Shim I, Kim HG, Lee CJ, Yoon BE. Proc Natl Acad Sci U S A. 2018 May 8;115(19):5004-5009. doi: 10.1073/pnas.1721187115. Epub 2018 Apr 24.

Development of a Low-cost, Comprehensive Recording System for Circadian Rhythm Behavior. Kwon J, Park MG, Lee SE, Lee CJ. Exp Neurobiol. 2018 Feb;27(1):65-75. doi: 10.5607/en.2018.27.1.65. Epub 2018 Feb 28.

Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocytes. Kwon J, An H, Sa M, Won J, Shin JI, Lee CJ. Exp Neurobiol. 2017 Feb;26(1):42-54. doi: 10.5607/en.2017.26.1.42. Epub 2017 Feb 9.

The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons. Ha GE, Lee J, Kwak H, Song K, Kwon J, Jung SY, Hong J, Chang GE, Hwang EM, Shin HS, Lee CJ, Cheong E. Nat Commun. 2016 Dec 19;7:13791. doi: 10.1038/ncomms13791.

EDUCATION

MAR 2014~ present     Ph.D. course, KU-KIST Graduate School of Converging Science and Technology, Seoul, Korea (Thesis advisor: Dr. Changjoon Justin Lee)

MAR 2006~ FEB 2011   B.A. College of Life science & Biotechnology, Korea University, Seoul, Korea

RESEARCH EXPERIENCE

NOV 2018~ Present     Researcher, Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Korea

FEB 2014~ NOV 2018    Researcher, Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul, Korea

RESEARCH INTERSTS

Neuron-glia interaction

Reactive astrocytes in hypothalamus, Obesity and metabolism

Learning and memory, ADHD

EXPERIMENTAL TECHNIQUES

Slice patch recording (Neuron), Cell patch (COS-7, HEK293T), Ca2+ Imaging

Confocal microscope imaging, Immunohistochemistry, Immunocytochemistry

Primary astrocyte culture, PCR, RT-PCR, DNA/RNA prep., Cloning

Stereotaxic surgery, Open field test, Elevate plus maze, Passive avoidance test, Eight arm radial maze

AWARDS

AUG 2018        BEST PRESENTATION AWARD at 21st KSBNS Annual Meeting

                    (Poster title: GABA from Reactive Astrocytes in Hypothalamus via Monoamine Oxidase B Causes and Exacerbates Obesity)

NOV 2016        Second Award at KIST-BSI Conference 2016

                    (Poster title: GABA from Reactive Astrocytes may Cause and Exacerbate Obesity)

SEP 2016        Second Award at 2016 Converging Conference for KIST-School Ties

                    (Poster title: GABA from Reactive Astrocytes may Cause and Exacerbate Obesity)

PUBLICATION

1.   NaHye Lee*, Moonsun Sa*, Yu Ri Hong, C. Justin Lee†, and JaeHyung Koo†. Fatty Acid Increases cAMP-dependent Lactate and MAO-B-dependent GABA Production in Mouse Astrocytes by Activating a Gαs Protein-coupled Receptor. Experimental Neurobiology, 2018 Oct;27(5):365-376. doi: 10.5607/en.2018.27.5.365.

2.   Jea Kwon, Heeyoung An, Moonsun Sa, Joungha Won, Jeong Im Shin, and C. Justin Lee. Orai1 and Orai3 in combination with stim1 mediate the  majority of store-operated calcium entry in astrocytes. Experimental Neurobiology, 2017 Feb;26(1):42-54. doi: 10.5607/en.2017.26.1.42.

EDUCATION

- 2007.03 ~ 2014.02 : B.A. in Yonsei University

- 2016.03 ~ Present : Ph.D Course (KU-KIST, Advisor: C. Justin Lee)

RESEARCH EXPERIENCES

- 2012.07 ~ 2013.06 Undergraduate Intern (Prof. Park in Yonsei University, Immunology)

- 2014.03 ~ 2014.08 Undergraduate Intern (Prof. Lee in Yonsei University, Bioinformatics)

- 2014.10 ~ 2016.02 Internship (Dr. C. Justin Lee's lab, Glioscience)

PUBLICATIONS

- Yong Kyoung Yoo, Jaekwang Lee, Jinsik Kim, Gangeun Kim, Sunpil Kim, Jeongyeon Kim, Heejung Chun, Jeong Hoon Lee, C. Justin Lee & Kyo Seon Hwang, Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor, Scientific Reports, 2016.

EXPERIMENTAL TECHNIQUES

- Imaging:

  Acute brain slice imaging (Calcium, Fura-2 and various sensors)

  2-photon brain slice imaging

- Behavior:

  Social behaviors (3-chamber test and five-trial social memory test)

  General behaviors (Open field test, novel object recognition and novel place recognition test)

  Fear-associated behavior (Passive avoidance test and contextual fear conditioning)

- Electrophysiology:

  Patch clamping (Cultured cell and acute brain slice)

- Molecular biology:

  Cloning, RT-PCR and Western blot

- Primary astrocyte culture and microporation

- Stereotaxic surgery :

  Guide cannulation (Microdialysis)

  Virus injection

- Immunohistochemistry (IHC) for mouse

- Microdialysis

AWARDS

- Outstanding presentation award (2018 5th SGFP Annual Conference)

EDUCATION

• 2010.03 ~ 2014.08 B.A. in Sookmyung Women’s University

• 2015.03 ~ KU-KIST Graduate School of Converging Science and Technology, Korea University

RESEARCH EXPERIENCES

• 2012.01 ~ 2012.12 Undergraduate intern (Prof. Jonghoon Park, Sookmyung Women’s University)

• 2013.06 ~ 2013.09 52^nd National Undergraduate Symposium on Biology (Aging & Senescence)

EXPERIMENTAL TECHNIQUES

• Electrophysiology: Cell patch, Slice patch

• Imaging: Calcium imaging, Intrinsic optical signal (IOS) imaging

• Molecular biology: Cloning, RT-PCR, Western blot, Biotinylation, Co-immunoprecipitation

• Cellular biology: Transfection, Microporation

• Primary culture: Astrocyte cell culture, Cochlea tissue culture

• Stereotaxic surgery (mouse)

• Immunohistochemistry, Immunocytochemistry

PUBLICATIONS

1. Han K, Lee M, Lim HK, Jang MW, Kwon J, Lee CJ, Kim SG, Suh M. Excitation-inhibition imbalance leads to alteration of neuronal coherence and neurovascular coupling under acute stress. J Neurosci. 2020 Oct 20:JN-RM-1553-20. doi: 10.1523/JNEUROSCI.1553-20.2020. PMID: 33087471.

    

2. Woo J, Jang MW, Lee J, Koh W, Mikoshiba K, Lee CJ. The molecular mechanism of synaptic activity-induced astrocytic volume transient. J Physiol. 2020 Jul 24. doi: 10.1113/JP279741. PMID: 32706443.

    

3. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ. Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Exp Neurobiol. 2019 Apr;28(2):183-215. doi: 10.5607/en.2019.28.2.183. Epub 2019 Apr 30. PMID: 31138989.

AWARDS

Oral Presentation:

• 2017 Brain Science Institute Symposium (The novel channel, AUDITORIN discloses a new class of hearing loss, auditory gliopathy)

Poster Presentation:

• 2018 Glia Meeting of the Korean Society for Glia Research (Human speech perception by AUDITORIN (TMEM43) in cochlear glia)

• 2019 7th SIPF-SIF International Workshop on: Structure and Function of Ion Channels and Transporters (AUDITORIN (TMEM43), A Novel Passive Conductance Channel of Cochlear Glia is Critical for Maintenance of Speech Discrimination)

EDUCATION 

- 2009.03 ~ 2014.03: B.S. in Korea University, Seoul, Republic of Korea
- 2015.03 ~ 2022.08: Ph.D course in KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea

ACADEMIC RESEARCH INTEREST

- Neuroscience (Neuron-glia interaction)
- Neurodegenerative diseases
- Systemic inflammation
- Reactive oxygen species (ROS)
-Drug development
- Post-traumatic stress disorder (PTSD)
- Emotions

EXPERIMENTAL TECHNIQUES 

- Electrophysiology: Cell patch, Slice patch 
- Imaging: Calcium imaging, Neurotransmitters, sensor probe
- Immunohistochemistry, Immunocytochemistry
- Molecular biology: Cloning, qRT-PCR, shRNA 
- Cellular biology: Transfection, Microporation 
- Astrocyte cell culture
- Behavior test (mouse) 
- Stereotaxic surgery (mouse, rat): virus injection, cannula surgery 
- Drug screening assay (H₂O₂, MAOB etc.)
- RNA-Sequencing

AWARD and HONORS

■ Oral Presentation award 
 - 2019 Korean Chemical Society, Division of Medicinal Chemistry; Best Presentation

■ Poster Presentation award
 - 2018 Glia Meeting of the Korean Society for Glia Research
 - 2019 Cold Spring Harbor Asia (CSHA) meeting, Francis Crick Symposium-Transforming Neurosciences:
Questions & Experiments
- 2021 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation Award
- 2022 Korean Society for Brain and Neural Sciences (KSBNS); Best Presentation Award
- 2022 IBS-UNIST Symposium; Best Presentation Award

PATENTS 

■ Composition for prevention or treatment of rheumatoid arthritis (10-2020-0120924)

■ NOVEL AMINOAROMATIC COMPOUNDS OR PHARMACEUTICALLY ACCEPTABLE SALT THEREOF AND PHARMACEUTICAL COMPOSITION FOR PREVENTION OR TREATMENT OF NEURODEGENERATIVE DISEASES COMPRISING THE SAME AS AN ACTIVE INGREDIENT (10-2021-0155076)

■ Prolonging lifespan with long-term administration of novel H2O2 scavenger (10-2022-0092661)

PUBLICATION

1. Won W, Choi HJ, Yoo JY, Kim D, Kim TY, Ju YH, Park KD, Lee H, Jung SY, Lee CJ. inhibiting peripheral and central MAO-B ameliorates both joint inflammation and cognitive impairment in rheumatoid arthritis. Exp Mol Med (in press)

2. An H, Lee H, Yang S, Won W, Lee CJ, Nam MH. Adenovirus-induced Reactive Astrogliosis Exacerbates the Pathology of Parkinson's Disease. Exp Neurobiol. 2021 Jun 30;30(3):222-231. doi: 10.5607/en21013. PMID: 34045369; PMCID: PMC8278136.

3. Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, Kim S, Jang MW, Won W, Park MG, Pae AN, Han S, Kim ST, Lee CJ. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med 53, 956–972 (2021).

4. Chun H, Im H, Kang YJ, Kim Y, Shin JH,Won W, Lim J, Ju Y, Park YM, Kim S, Lee SE, Lee J, Woo J, Hwang Y, Cho H, Jo S, Park JH, Kim D, Kim DY, Seo JS, Gwag BJ, Kim YS, Park KD, Kaang BK, Cho H, Ryu H, Lee CJ. Severe reactive astrocytes precipitate pathological hallmarks of Alzheimer's disease via H2O2- production. Nat Neurosci. 2020 Dec;23(12):1555-1566. doi: 10.1038/s41593-020-00735-y. Epub 2020 Nov 16. PMID: 33199896.

5. Nam MH, Cho J, Kwon DH, Park JY, Woo J, Lee JM, Lee S, Ko HY, Won W, Kim RG, Song H, Oh SJ, Choi JW, Park KD, Park EK, Jung H, Kim HS, Lee MC, Yun M, Lee CJ, Kim HI. Excessive Astrocytic GABA Causes Cortical Hypometabolism and Impedes Functional Recovery after Subcortical Stroke.Cell Rep. 2020 Jul 7;32(1):107861. doi: 10.1016/j.celrep.2020.107861. Erratum in: Cell Rep. 2020 Jul 21;32(3):107975. PMID: 32640227.

6. Nam MH*, Won W*, Han KS, Lee CJ. Signaling mechanisms of μ-opioid receptor (MOR) in the hippocampus: disinhibition versus astrocytic glutamate regulation. Cell Mol Life Sci. 2021 Jan;78(2):415-426. doi: 10.1007/s00018-020-03595-8. Epub 2020 Jul 15. PMID: 32671427.

- Ph.D course (KU-KIST)

-Glia-neuron interaction, Neurodegeneration and Neuroinflammation.

- wwj@ibs.re.kr

Educational background

- 2016/09 ~ Present, Ph.D. candidate in KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea (Advisor: C. Justin, Lee)

- 2010/03 ~ 2016/08, B.E. in Biomedical engineering, Korea University, Seoul, Republic of Korea

- 2012/05 ~ 2014/02, KATUSA soldier program (U.S. Army Garrison Casey, South Korea)

Research Experiences

- 2014/09 ~ 2016/05, Undergraduate internship, Prof. Sang-Hoon Lee’s iBML lab, Korea University.

Field of interest

- Targeting astrocytes as a novel therapeutic target for treating epilepsy.

- Physiological and pathological function of TRIM9 in astrocytes.

- Modulation of synaptic transmission by astrocytes.

- Mechanosensitive calcium channels on radial glial cells.

EXPERIMENTAL TECHNIQUES

- Calcium imaging, GABA imaging

- Behavior test (Passive avoidance test, Open field test, novel object recognition and novel place recognition test)

 - Electrophysiology: Patch clamping (Cultured cell and acute brain slice), Field recording

- Molecular biology: Cloning, RT-PCR and Western blot

- Primary astrocyte culture

- ICC and IHC

- Virus injection

Publications

-Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive calcium channel. Park MG, Jang H, Lee SH, Lee CJ, Exp Neurobiol 2017 Apr;26(2):71-81. (2017)

- Development of a low-cost, comprehensive recording system for circadian rhythm behavior. Jea Kwon, Min Gu Park, Seung Eun Lee, and C. Justin Lee. Exp Neurobiol. 2019 Feb;28(1):30-42 (2018)

- Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient. Woo J, Han YE, Koh W, Won J, Park MG, An H, Lee CJ, Exp Neurobiol 2018 Feb 27;27(1):65-75; (2018)

- Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Han YE, Kwon J, Won J, An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215 (2019)

Awards

- 2017/03 ~ Present, Global Ph.D. Fellowship program from National Research Foundation of Korea

EDUCATION

-2013.03 ~ 2017.08 B.A in Seoul National University

-2017.09 ~ Present Ph.D candidate in University of Science and Technology(UST)

RESEARCH EXPERIENCE

-2016.06 ~ 2016.08 Students intern researcher program(학생인턴연구원) in School of Medicine, Seoul National University.

-2017.03 ~ 2017.08 Undergraduate intern in Dr. C. Justin Lee lab, Korea Institute of Science and Technology (KIST)

EXPERIMENTAL TECHNIQUES

-Molecular biology: Cloning, RT-PCR

-Cellular biology: Transfection, Microporation

-Astrocyte cell culture (+ Long-term culture)

-Stereotaxic surgery (mouse)

-Immunohistochemistry

-Imaging: Calcium imaging in vitro

-Electrophysiology: Cell patch, Slice patch

-Behavior test: Passive avoidance test, Open field test, Novel object (Place) recognition test, Context fear conditioning test, Elevated plus maze test, Forced swim test

AWARDS

-Poster presentation

2018 Glia Meeting of the Korean Society for Glia Research (Charcterization of hGFAP-CreERT2 [Tg(GFAP-Cre/ERT2)13Kdmc] in various brain region)

PUBLICATION

-Koh, W., Park, Y. M., Lee, S. E., & Lee, C. J. (2017). AAV-mediated astrocyte-specific gene expression under human ALDH1L1 promoter in mouse thalamus. Experimental neurobiology, 26(6): 350-361.

- Park, Y. M., Chun, H., Shin, J. I., & Lee, C. J. (2018). Astrocyte Specificity and Coverage of hGFAP-CreERT2 [Tg (GFAP-Cre/ERT2) 13Kdmc] Mouse Line in Various Brain Regions. Experimental neurobiology, 27(6): 508-525.

Education

PhD course, Department of Basic Science, IBS School in University of Science and Technology (UST), KOREA (2017.03-present)

M.S, Department of Mathematics, Konkuk University, KOREA (2013.09-2015.08)

B.S, Department of Mathematics, Konkuk University, KOREA (2009.03-2013.08)

Research experiences

2015.07~2016.08 rare cancer branch in National Cancer Center (NCC)

2016.10~2018.05 in Korea Institute of Radiological and Medical Sciences (KIRAMS)

Awards

2015 Poster Presentation Award from Korean Society for Industrial and Applied Mathematics.

(Mathematical modeling of Glioblastoma cell type specific about FLIP-induced TRAIL resistance)

2014 Poster Presentation Award from Korean Society of Mathematical Biology.

(Mathematical modeling of the AKT-mTOR-p70S6K pathway in regulation of the control of FLIP and TRAIL-induced apoptosis in cancer)

Scholarships

2018-1^st Assistant scholarship from University of Science and Technology (UST), Korea

2013-2^nd ~2015-1^st Graduate teaching Scholarship from Konkuk University, KOREA.

MRIDULA NEERAJ KUMAR BHALLA – CV

Contact details - +82-010-7286-9608, +91 97667-11187 ; bmridula158@ibs.re.kr

Date of Birth                          :               15^th August 1996

Nationality                              :                Indian

Research Interest

Astrocytes and CNS demyelination, Astrocytes in aging

Education

Research Experience

Publication

Research Techniques

Awards, Honors, and Scholarships

Curricular and Extracurricular experience

JinHyeong Joo, Integrated Ph.D course

E-mail : jhjoo@ibs.re.kr (official) / wnwlsgud0430@gmail.com (private)

Tel : +82) 010-6847-3680

RESEARCH INTEREST

• Neurodegenerative Disease & Memory in Astrocyte

   

EDUCATION

• [2021.01 ~ Present] Integrated Ph.D course at Univesity of Science & Technology (UST), Korea,

• [2021.01 ~ Present] Dr. C. Justin Lee’s Lab, Institute of Basic Science (IBS) School of UST,

• [2014.03 ~ 2019.02] B.E. in Ajou University, Applied Chemistry & Biological Engineering

   

RESEARCH EXPERIENCES

• [2020.03 ~ 2020.11] Reseacher in Immunobiome, Inc. in POSTECH biotech center, Pohang

• [2019.08. ~ 2019.08] Internship in POSTECH IRT lab. (Shin Hyeog Im’s lab), Pohang

• [2018.07 ~ 2018.09] Internship in Purification process in CJ 제일제당 laboratory, Suwon

RESEARCH TECHNIQUES

Mouse handling

• Oral gavage, i.p./s.c. injection, Blood collection (Heart, Orbital), Manual heart perfusion, mouse breeding

Behavior Test

• Sociability test, Social novelty test via 3-chamber arena, Marble burying test for evaluate repetitive behavior

Molcular work

• DNA / RNA prep, Gel electrophoresis, PCR, qRT-PCR, genotyping,

Cell work

• FACS

Joon-Ho Kwon

Curriculum Vitae ( Updated: Feb 25, 2022 )

IBS ( Institute for Basic Science )

Center for Cognition and Sociality

55, Expo-ro, Yuseong-gu, Daejeon, 34126 Republic of Korea
Mobile : +82-10-4189-9405

Work Email: kwonjoonho@unist.ac.kr

Feb. 2015              Chonnam National University                                                                                                          Gwangju, Korea

                                 Department of Biological Sciences

                                 B.S. in Biological Sciences

Feb. 2017              Korea University                                                                                                                                        Seoul, Korea 

                                 Division of Life Sciences

                                 Thesis: Physiological interaction between copper and zinc in aspergillus fumigatus.

                                 Advisor: Prof. Cheol-Won Yun

                                 M.S. in Life Sciences

Mar. 2022             UNIST (Ulsan National Institute of Science and Technology)                                                          Ulsan, Korea

~ Present               Department of Biomedical Engineering

Co-Advisor: Dr. C. Justin Lee, Prof. Yoon-Kyoung Cho

Ph.D. course

1. Master’s student, Korea University, Laboratory of  Molecular Microbiology, ( Jan. 2015 ~ Dec. 2016 )

2. Technical Research Personnel, Seoul National University College of Medicine, Laboratory of Molecular Imaging and Therapy ( Jan. 2017 ~ Mar. 2020 )

3. Researcher, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Infection and Immunity Research Laboratory ( Apr. 2020 ~ Jun. 2021 )

4. Researcher, Institute for Basic Science (IBS), Cognitive Glioscience group, Director: Dr. C. Justin Lee’s Lab, Center for Cognition and Sociality ( Jul. 2021 ~ Feb. 2022 )

5. Ph.D. Student,UNIST-IBS Neuroscience scholarship ( Mar. 2022 ~ Present )

1. Kim, M. J., Lee, C. H., Lee, Y., Youn, H., Kang, K. W., Kwon, J. H, ... & Chung, J. K. (2019). Glucose-6-phosphatase Expression–Mediated [18 F] FDG Efflux in Murine Inflammation and Cancer Models. Molecular imaging and biology, 21(5), 917-925.

2. Kang, S., Seo, H., Moon, H. S., Kwon, J. H., Park, Y. S., & Yun, C. W. (2020). The Role of Zinc in Copper Homeostasis of Aspergillus fumigatus. International journal of molecular sciences, 21(20), 7665.

    

1. Joon-Ho Kwon, Cheol-Won Yun, "Physiological interaction between copper and zinc in aspergillus fumigatus", International Meeting of the Federation of Korean Microbiological Societies (2016) – poster

    

    

Month/Year        Title                                                                                          Institution                              

03/2015-09/2015    BK21 PLUS Scholarship (National Funding for Life Sciences)       Korea University

09/2015-09/2016    Teaching Assistant Scholarship                                                         Korea University

General mouse works ( Organ dissection, maintenance, genotyping ), Cell culture (  Fungi, Various cancer cells, primary Thyroid cancer cells ), Cloning, Transfection, Transduction, PCR, Northern blot, RT-qPCR, Western blot, Pull-down assay, Luciferase assay, Cryosection and IHC( Immunohistochemistry ), IF( immunofluorescence ), Confocal Microscopy, Tumor ( Breast, Brain ) modeling, Stereotaxic injection, Small animal imaging test ( PET/CT, IVIS ), FACS, ICP-OES.

Jiwoon Lim

Research Interest

Astrocyte’s role in synapse, memory, and disease

Education

Research Experience

Publication

Poster Exhibition

Lim, J.*, Jee, S., Kwon J., Sa M. & Lee, C. J. (2020). Trifluoperazine (TFP) elevates intracellular Ca2+ level and maintains the open state of the store-operated calcium entry (SOCE) in astrocyte. The Korean Society for Brain and Neural Sciences, Seoul, Korea of Republic

Research Techniques

Awards, Honors, and Scholarships