EDUCATION
Undergraduate, 2001/3~2005/2, Biological Sciences, Korea University
Graduate, 2005/8~2015/8, Interdisciplinary Program in Neuroscience, Seoul National University
RESEARCH EXPERIENCES
2010/7~2014/06, Researcher, KIST
2014/07~2015/6, Visiting Graduate student, KIST
2016/7-present, Post doctoral fellow, KIST
EXPERICENED RESEARCH SKILLS
Slice Patch (Neuron and Astrocyte), Immunohistochemistry, Confocal imaging, Behavioral Test (passive avoidance test, open field test, fear conditioning, elevated plus maze), Molecular Biological Techniques (Cloning, Subcloning and RT-PCR), Stereotaxic Surgery, Cell Culture (Primary astrocyte, Primary neuron, and Cell line)
PUBLICATIONS
1. Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors. Park H, Han KS, Seo J, Lee J, Dravid SM, Woo J, Chun H, Cho S, Bae J, An H, Koh W, Yoon BE, Berlinguer-Palmini R, Mannaioni G, Traynelis SF, Bae Y, Choi SY, Lee C. Mol Brain. 2015 Feb 3;8(1):7 2. Glial GABA, synthesized by monoamine oxidase B, mediates tonic inhibition Bo-Eun Yoon, JunsungWoo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, JooOkMin, Soo-Jin Oh, Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Yong Chul Bae and C. Justin Lee. J Physiol. 2014 Sep 19. 3. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease, Seonmi Jo, Oleg Yarishkin, Yu Jin Hwang, Ye Eun Chun, Mijeong Park, Dong Ho Woo, Jin Young Bae, Taekeun Kim, Jaekwang Lee, Heejung Chun, Hyun Jung Park, Da Yong Lee, Jinpyo Hong, Hye Yun Kim, Soo-Jin Oh, Seung Ju Park, Hyo Lee, Bo-Eun Yoon, YoungSoo Kim, Yong Jeong, Insop Shim, Yong Chul Bae, Jeiwon Cho, Neil W Kowall, Hoon Ryu, Eunmi Hwang, Daesoo Kim & C Justin Lee Nat Med. 2014 Jun 29. 4. AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia. Lee YS, Choi SL, Jun H, Yim SJ, Lee JA, Kim HF, Lee SH, Shim J, Lee K, Jang DJ, Kaang BK. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15520-5. Epub 2012 Sep 4. 5. PKA-activated ApAF-ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation. Lee JA, Lee SH, Lee C, Chang DJ, Lee Y, Kim H, Cheang YH, Ko HG, Lee YS, Jun H, Bartsch D, Kandel ER, Kaang BK. J Cell Biol. 2006 Sep 11;174(6):827-38. 6. Regulation of ApC/EBP mRNA by the Aplysia AU-rich element-binding protein, ApELAV, and its effects on 5-hydroxytryptamine-induced long-term facilitation. Yim SJ, Lee YS, Lee JA, Chang DJ, Han JH, Kim H, Park H, Jun H, Kim VN, Kaang BK. J Neurochem. 2006 Jul;98(2):420-9. 7. SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses.Ko J, Kim S, Chung HS, Kim K, Han K, Kim H, Jun H, Kaang BK, Kim E. Neuron. 2006 Apr 20;50(2):233-45.
Postdoc. | |
From 2007.9 to 2009.7 | |
UC Berkeley, Moo-ming Poo's lab. | |
Contact : mythhero@gmail.com | |
Currently at KBRI (선임연구원) |
Korea University, College of Medicine , Department of Anesthesiology and Pain Medicine, Professor | |
hwshin99@yahoo.com |
Education
March
2002 - August 2010 B.A. Korea University
ü Major:
Business & Administration
ü Minor:
Law
September 2013 - present Ph.D. course, KU-KIST Graduate School of Converging
Science and Technology
ü Major: NBIT (Advanced
educated in Neuroscience)
Researcher,
Center for Cognition and Sociality, Institute for Basic Science (IBS)
Research
Experiments
October
2012 - August 2013 Internship at KIST
ü Center
for Functional Connectomics
Experiment
Techniques
Slice
patch-clamp recording (Neuron, Astrocyte), Cell patch (Astrocyte, HEK293T),
Sniffer patch, Ca2+ imaging, Primary astrocyte culture, Primary
neuron culture, Immunohistochemistry, Immunocytochemistry, Confocal microscope
imaging, SIM imaging, Imaris 3D rendering, PCR, RT-PCR, DNA/RNA prep, Cloning, Sub-cloning,
Stereotaxic surgery (Mouse, Monkey)
Research Interests
ü
Transdiffernentiation,
Treating Spinal Cord Injury (SCI) and neurodegenerative diseases with direct
reprogramming of reactive astrocyte into neuron
ü
Bestrophin1
channel expression related synaptic property
Publications
1.
Tweety-homolog (Ttyh)
Family Encodes the Pore-forming Subunits of the Swelling-dependent
Volume-regulated Anion Channel (VRACswell) in the Brain, Han YE, Kwon J, Won J,
An H, Jang MW, Woo J, Lee JS, Park MG, Yoon BE, Lee SE, Hwang EM, Jung
JY, Park H, Oh SJ, Lee CJ, Exp Neurobiol. 2019 Apr;28(2):183-215
2.
Pharmacological
Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between
Synaptic Activity and Astrocytic Volume Transient, Junsung Woo, Young-Eun Han,
Wuhyun Koh, Joungha Won, Min Gu Park, Heeyoung
An, C. Justin Lee, Exp Neurobiol. 2019 Feb;28(1):30-42
3.
Expression of
µ-Opioid Receptor in CA1 Hippocampal Astrocytes, Min-Ho Nam, Kyung-Seok Han,
Jaekwang Lee, Jin Young Bae, Heeyoung An,
Seahyung Park, Soo-Jin Oh, Eunju Kim, Eunmi Hwang, Yong Chul Bae, C. Justin
Lee, Exp Neurobiol. 2018 Apr; 27(2): 120–128.
4.
Control of motor
coordination by astrocytic tonic GABA release through modulation of
excitation/inhibition balance in cerebellum, Woo J, Min JO, Kang DS, Kim YS,
Jung GH, Park HJ, Kim S, An H, Known
J, Kim J, Shim I, Kim HG, Lee CJ, Yoon BE, Proc Natl Acad Sci U S A. 2018 Apr
24. pii: 1721187115. do (2018)
5.
Cografting
astrocytes improves cell therapeutic outcomes in a Parkinson`s disease model,
Song JJ, Oh SM, Kwon OC, Wulansari N, Lee HS, Chang MY, Lee E, Sun W, Lee SE,
Chang S, An H, Lee CJ, Lee SH, J
Clin Invest 2018 Jan 2;128(1):463-482. (2018)
6.
Astrocytic water
channel aquaporin-4 modulates brain plasticity in both mice and humans: a
potential gliogenetic mechanism underly, J Woo, JE Kim, JJ Im, J Lee, HS Jeong,
S Park, S-Y Jung, H An, S Yoon, SM
Lim, S Lee, J Ma, EY Shin, Y-E Han, B Kim, EH Lee, L Feng, H Chun, B-E Yoon, I
Kang, SR Dager, IK Lyoo and CJ Lee, Molecular Psychiatry advance online
publication 27 June 2017; doi:10.1038/mp.2017.113 (2017)
7.
Orai1 and Orai3
in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry
in Astrocytes, Jea Kwon, Heeyoung An,
Moonsun Sa, Joungha Won,Jeong Im Shin and C. Justin Lee, Exp Neurobiol 2017
Feb;26(1):42-54. (2017)
8.
Differential Cellular
Tropism of Lentivirus and Adeno-Associated Virus in the Brain of Cynomolgus
Monkey, An H, Cho DW, Lee SE, Yang
YS, Han SC, Lee CJ, Exp Neurobiol 2016 Feb;25(1):48-54. doi:
10.5607/en.2016.25.1.48. (2016)
9.
Networked neural
spheroid by neuro-bundle mimicking nervous system created by topology effect,
Jeong GS, Chang JY, Park JS, Lee SA, Park D, Woo J, An H, Lee CJ, Lee SH, Mol Brain 2015 Mar 22;8(1):17. doi:
10.1186/s13041-015-0109-y. (2015)
10. Channel-mediated
astrocytic glutamate modulates hippocampal synaptic plasticity by activating
postsynaptic NMDA receptors, Park H, Han KS, Seo J, Lee J, Dravid SM, Woo J,
Chun H, Cho S, Bae J, An H, Koh W,
Yoon BE, Berlinguer-Palmini R, Mannaioni G, Traynelis SF, Bae Y, Choi SY, Lee
CJ, Mol Brain 2015 Feb 3;8(1):7. (2015)
11. Glial
GABA, synthesized by monoamine oxidase B, mediates tonic inhibition. Bo-Eun
Yoon, JunsungWoo, Ye-Eun Chun, Heejung Chun, Seonmi Jo, Jin Young Bae, Heeyoung An, JooOkMin, Soo-Jin Oh,
Kyung-Seok Han, Hye Yun Kim, Taekeun Kim, Young Soo Kim, Yong Chul Bae and C.
Justin Lee, J Physiol. 2014 Sep 19. DOI: 10.1113 (2014)
Awards
1.
Oral
presentation:
i.
Direct cell
reprogramming of reactive astrocytes into neurons in non-human primate, 2014
KU-KIST symposium
2.
Poster
Presentations:
i.
Transdifferentiation
of scar-forming reactive astrocytes into functional neurons causes motor
recovery after spinal cord injury, 2019 Korea Society for Gene and Cell Therapy
ii.
Cell direct
reprogramming of reactive astrocytes into neurons in non-human primates. 2015
Glia Meeting of the Korean Society for Glia Research