시설/장비
SPR (표면플라즈몬 공명장치)
Biacore T200
표면 플라즈몬 공명 장치
- 제조사Cytiva
- 응용분야약물발견, 항체특성, 단백질학, 면역원성, 생물 치료제 개발 및 생산, 다양한 생명과학 연구 응용분야
- 주용도Sensor Chip을 이용하여 분자간의 상호작용을 측정
장비사양
- Affinity rangefM to mM
- Analysis temperature4°C to 45°C (maximum 20°C below ambient temperature)
- Analysis time per cycleTypically 2 to 15 min
- Application1Kinetics/affinity characterization, kinetics/affinity screening, single-cycle kinetics, LMW interaction analysis, fragment screening, epitope mapping1, immunogenicity, concentration analysis, calibration-free concentration analysis, thermodynamics, comparability, sample recovery MS
- Association rate constant (ka)Proteins 103to 3 x 109M-1s-1LMW molecules 103to 5 x 107M-1s-1
- Automation48h unattended operation
- Baseline drift0.3 RU/min
- Baseline noise0.03 RU (RMS)
- Buffer selectionAutomatic switching between four buffers
- Capacity1 x 96- or 384-well microplate and up to 33 reagent vials or 78 vials for samples and reagents
- Concentration rangeSample concentration → 1 pM
- Dissociation rate constant (kd)10-5to 1 s-1
- Flow cell volume60 nL
- Flow rate1 to 100 μL/min
- Injection volume2 to 350 μL
- Instrument dimensions, W x H x D600 x 690 x 615 mm
- Mains requirementProcessing unit: Autorange 100 to 240 V~, 50/60 Hz
- Molecular weight detectionNo lower limit for organic molecules
- Number of flow cells4
- Power consumptionProcessing unit: max. 400 VA
- Refractive index1.33 to 1.40
- Sample storage temperature4°C to 45°C (maximum 15°C below ambient temperature)
- Sample typeSmall-molecule drug candidates to high-molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells, and viruses) in various sample environments (e.g., in DMSO-containing buffers, plasma, and serum)
- Sample volume min.Sample volume Injection volume plus 20 to 50 µL (application dependent)
- Weight60 kg
- Flow cell height40 µmµm
이용료
| 구분 | 연구시설장비명 | 단가 | 이용료(원) |
|---|---|---|---|
| SPR | Biacore T200 (표면 플라즈몬 공명 장치) |
시간 | 40,000 |
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